| Revue: | The journal of venomous animals and toxins |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000298765 |
| ISSN: | 0104-7930 |
| Autores: | Borja-Oliveira, C.R1 Soares, A.M2 Zamuner, S.R Hyslop, S Giglio, J.R3 Prado-Franceschi, J Rodrigues-Simioni, L |
| Instituciones: | 1Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Campinas, Sao Paulo. Brasil 2Universidade de Ribeirao Preto, Departamento de Biotecnologia, Ribeirao Preto, Sao Paulo. Brasil 3Universidade de Sao Paulo, Faculdade de Medicina de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil |
| Año: | 2002 |
| Volumen: | 8 |
| Número: | 1 |
| Paginación: | 88-101 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental |
| Resumen en inglés | Snake venoms frequently vary in composition. In this work, we compared the neurotoxic and myotoxic activities of 16 lots of Bothrops neuwiedii venoms from different regions of Brazil, using chick biventer cervicis preparations. The neuromuscular blockade varied from 2% to 100% after 120 min incubation with venoms (50 mug/ml). In all cases, this blockade was irreversible and concentration-dependent; at low concentrations (10-20 mug/ml), 15 of the 16 venom lots failed to abolish responses to acetylcholine (110 muM), but blocked responses to KCl (13.4 muM), and induced contracture. At 5-20 mug/ml, the most active venom totally blocked twitch-tension without affecting responses to acetylcholine and KCl. Polyacrylamide gel electrophoresis for basic proteins showed that the most active samples contained a band that was absent in the less active venoms. These results indicate that there may be considerable intraspecific variation in the neurotoxic activity of B. neuwiedii venoms, whereas myotoxic activity is less variable |
| Disciplinas: | Biología, Medicina |
| Palabras clave: | Toxicología, Reptiles, Veneno de víbora, Variación intraespecífica, Miotoxicidad, Neurotoxicidad, Bloqueo neuromuscular, Bothrops neuwiedii, Viperidae |
| Keyword: | Biology, Medicine, Toxicology, Reptiles, Snake venom, Intraspecific variation, Myotoxicity, Neurotoxicity, Neuromuscular blockade, Bothrops neuwiedii, Viperidae |
| Texte intégral: | Texto completo (Ver HTML) |
