Intraspecific variation in neurotoxic and myotoxic activities of Bothrops neuwiedii venoms



Título del documento: Intraspecific variation in neurotoxic and myotoxic activities of Bothrops neuwiedii venoms
Revista: The journal of venomous animals and toxins
Base de datos: PERIÓDICA
Número de sistema: 000298765
ISSN: 0104-7930
Autores: 1
2


3

Instituciones: 1Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Campinas, Sao Paulo. Brasil
2Universidade de Ribeirao Preto, Departamento de Biotecnologia, Ribeirao Preto, Sao Paulo. Brasil
3Universidade de Sao Paulo, Faculdade de Medicina de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil
Año:
Volumen: 8
Número: 1
Paginación: 88-101
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés Snake venoms frequently vary in composition. In this work, we compared the neurotoxic and myotoxic activities of 16 lots of Bothrops neuwiedii venoms from different regions of Brazil, using chick biventer cervicis preparations. The neuromuscular blockade varied from 2% to 100% after 120 min incubation with venoms (50 mug/ml). In all cases, this blockade was irreversible and concentration-dependent; at low concentrations (10-20 mug/ml), 15 of the 16 venom lots failed to abolish responses to acetylcholine (110 muM), but blocked responses to KCl (13.4 muM), and induced contracture. At 5-20 mug/ml, the most active venom totally blocked twitch-tension without affecting responses to acetylcholine and KCl. Polyacrylamide gel electrophoresis for basic proteins showed that the most active samples contained a band that was absent in the less active venoms. These results indicate that there may be considerable intraspecific variation in the neurotoxic activity of B. neuwiedii venoms, whereas myotoxic activity is less variable
Disciplinas: Biología,
Medicina
Palabras clave: Toxicología,
Reptiles,
Veneno de víbora,
Variación intraespecífica,
Miotoxicidad,
Neurotoxicidad,
Bloqueo neuromuscular,
Bothrops neuwiedii,
Viperidae
Keyword: Biology,
Medicine,
Toxicology,
Reptiles,
Snake venom,
Intraspecific variation,
Myotoxicity,
Neurotoxicity,
Neuromuscular blockade,
Bothrops neuwiedii,
Viperidae
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