| Revue: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459473 |
| ISSN: | 0074-0276 |
| Autores: | Giordano, Ana Luisa Perini Leme1 Pontes, Lais1 Beraquet, Caio Augusto Gualtieri1 Lyra, Luzia1 Schreiber, Angelica Zaninelli1 |
| Instituciones: | 1Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Campinas, Sao Paulo. Brasil |
| Año: | 2023 |
| Volumen: | 118 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) allows rapid pathogen identification and potentially can be used for antifungal susceptibility testing (AFST). OBJECTIVES We evaluated the performance of the MALDI-TOF MS in assessing azole susceptibility, with reduced incubation time, by comparing the results with the reference method Broth Microdilution. METHODS Resistant and susceptible strains of Candida (n = 15) were evaluated against fluconazole and Aspergillus (n = 15) against itraconazole and voriconazole. Strains were exposed to serial dilutions of the antifungals for 15 h. Microorganisms’ protein spectra against all drug concentrations were acquired and used to generate a composite correlation index (CCI) matrix. The comparison of autocorrelations and cross-correlations between spectra facilitated by CCI was used as a similarity parameter between them, enabling the inference of a minimum profile change concentration breakpoint. Results obtained with the different AFST methods were then compared. FINDINGS The overall agreement between methods was 91.11%. Full agreement (100%) was reached for Aspergillus against voriconazole and Candida against fluconazole, and 73.33% of agreement was obtained for Aspergillus against itraconazole. MAIN CONCLUSIONS This study demonstrates MALDI-TOF MS’ potential as a reliable and faster alternative for AFST. More studies are necessary for method optimisation and standardisation for clinical routine application |
| Disciplinas: | Medicina |
| Palabras clave: | Farmacología, Bioquímica, Antifúngicos, Pruebas de susceptibilidad, Azoles, Espectrometría de masas, Indice de correlación compuesta, Candida, Aspergillus |
| Keyword: | Pharmacology, Biochemistry, Antifungal agents, Susceptibility tests, Azoles, Mass spectrometry, Composite correlation index, Candida, Aspergillus |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
