Revista: | Memorias do Instituto Oswaldo Cruz |
Base de datos: | PERIÓDICA |
Número de sistema: | 000459473 |
ISSN: | 0074-0276 |
Autores: | Giordano, Ana Luisa Perini Leme1 Pontes, Lais1 Beraquet, Caio Augusto Gualtieri1 Lyra, Luzia1 Schreiber, Angelica Zaninelli1 |
Instituciones: | 1Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Campinas, Sao Paulo. Brasil |
Año: | 2023 |
Volumen: | 118 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) allows rapid pathogen identification and potentially can be used for antifungal susceptibility testing (AFST). OBJECTIVES We evaluated the performance of the MALDI-TOF MS in assessing azole susceptibility, with reduced incubation time, by comparing the results with the reference method Broth Microdilution. METHODS Resistant and susceptible strains of Candida (n = 15) were evaluated against fluconazole and Aspergillus (n = 15) against itraconazole and voriconazole. Strains were exposed to serial dilutions of the antifungals for 15 h. Microorganisms’ protein spectra against all drug concentrations were acquired and used to generate a composite correlation index (CCI) matrix. The comparison of autocorrelations and cross-correlations between spectra facilitated by CCI was used as a similarity parameter between them, enabling the inference of a minimum profile change concentration breakpoint. Results obtained with the different AFST methods were then compared. FINDINGS The overall agreement between methods was 91.11%. Full agreement (100%) was reached for Aspergillus against voriconazole and Candida against fluconazole, and 73.33% of agreement was obtained for Aspergillus against itraconazole. MAIN CONCLUSIONS This study demonstrates MALDI-TOF MS’ potential as a reliable and faster alternative for AFST. More studies are necessary for method optimisation and standardisation for clinical routine application |
Disciplinas: | Medicina |
Palabras clave: | Farmacología, Bioquímica, Antifúngicos, Pruebas de susceptibilidad, Azoles, Espectrometría de masas, Indice de correlación compuesta, Candida, Aspergillus |
Keyword: | Pharmacology, Biochemistry, Antifungal agents, Susceptibility tests, Azoles, Mass spectrometry, Composite correlation index, Candida, Aspergillus |
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