Leishmania enriettii visceralises in the trachea, lungs, and spleen of Cavia porcellus



Título del documento: Leishmania enriettii visceralises in the trachea, lungs, and spleen of Cavia porcellus
Revue: Memorias do Instituto Oswaldo Cruz
Base de datos: PERIÓDICA
Número de sistema: 000452396
ISSN: 0074-0276
Autores: 1
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Instituciones: 1Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas, Belo Horizonte, Minas Gerais. Brasil
2Fundacao Oswaldo Cruz, Instituto Rene Rachou, Belo Horizonte, Minas Gerais. Brasil
3Universidade de Sao Paulo, Faculdade de Medicina, Sao Paulo. Brasil
Año:
Volumen: 117
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Leishmania (Mundinia) enriettii is a species commonly found in the guinea pig, Cavia porcellus. Although it is a dermotropic species, there is still an uncertainty regarding its ability to visceralise during Leishmania life cycle. OBJECTIVE Here, we investigated the ability of L. enriettii (strain L88) to visceralise in lungs, trachea, spleen, and liver of C. porcellus, its natural vertebrate host. METHODS Animals were infected sub-cutaneously in the nose and followed for 12 weeks using histological (hematoxilin-eosin) and molecular tools (polymerase chain reaction-restriction fragment length polymorphism - PCR-RFLP). To isolate parasite from C. porcellus, animals were experimentally infected for viscera removal and PCR typing targeting hsp70 gene. FINDINGS Histological analysis revealed intense and diffuse inflammation with the presence of amastigotes in the trachea, lung, and spleen up to 12 weeks post-infection (PI). Molecular analysis of paraffin-embedded tissues detected parasite DNA in the trachea and spleen between the 4th and 8th weeks PI. At the 12th PI, no parasite DNA was detected in any of the organs. To confirm that the spleen could serve as a temporary site for L. enriettii, we performed additional in vivo experiments. During 6th week PI, the parasite was isolated from the spleen confirming previous histopathological and PCR observations. MAIN CONCLUSION Leishmania enriettii (strain L88) was able to visceralise in the trachea, lung, and spleen of C. porcellus
Disciplinas: Medicina
Palabras clave: Parasitología,
Mamíferos,
Vísceras,
Histopatología,
Leishmania enriettii,
Cobayos,
Cavia porcellus
Keyword: Parasitology,
Mammals,
Viscera,
Histopathology,
Leishmania enriettii,
Guinea pigs,
Cavia porcellus
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