| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000452396 |
| ISSN: | 0074-0276 |
| Authors: | Alves Sobrinho, Ednéia Venâncio1 Pinheiro, Lucélia de Jesus1 Paranaíba, Larissa Ferreira1 Fontes, Igor Campos1 Parreiras, Patrícia Martins2 Gontijo, Nelder Figueiredo1 Tafuri, Wagner Luiz1 Laurenti, Márcia Dalastra3 Soares, Rodrigo Pedro2 |
| Institutions: | 1Universidade Federal de Minas Gerais, Instituto de Ciencias Biologicas, Belo Horizonte, Minas Gerais. Brasil 2Fundacao Oswaldo Cruz, Instituto Rene Rachou, Belo Horizonte, Minas Gerais. Brasil 3Universidade de Sao Paulo, Faculdade de Medicina, Sao Paulo. Brasil |
| Year: | 2022 |
| Volumen: | 117 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Leishmania (Mundinia) enriettii is a species commonly found in the guinea pig, Cavia porcellus. Although it is a dermotropic species, there is still an uncertainty regarding its ability to visceralise during Leishmania life cycle. OBJECTIVE Here, we investigated the ability of L. enriettii (strain L88) to visceralise in lungs, trachea, spleen, and liver of C. porcellus, its natural vertebrate host. METHODS Animals were infected sub-cutaneously in the nose and followed for 12 weeks using histological (hematoxilin-eosin) and molecular tools (polymerase chain reaction-restriction fragment length polymorphism - PCR-RFLP). To isolate parasite from C. porcellus, animals were experimentally infected for viscera removal and PCR typing targeting hsp70 gene. FINDINGS Histological analysis revealed intense and diffuse inflammation with the presence of amastigotes in the trachea, lung, and spleen up to 12 weeks post-infection (PI). Molecular analysis of paraffin-embedded tissues detected parasite DNA in the trachea and spleen between the 4th and 8th weeks PI. At the 12th PI, no parasite DNA was detected in any of the organs. To confirm that the spleen could serve as a temporary site for L. enriettii, we performed additional in vivo experiments. During 6th week PI, the parasite was isolated from the spleen confirming previous histopathological and PCR observations. MAIN CONCLUSION Leishmania enriettii (strain L88) was able to visceralise in the trachea, lung, and spleen of C. porcellus |
| Disciplines: | Medicina |
| Keyword: | Parasitología, Mamíferos, Vísceras, Histopatología, Leishmania enriettii, Cobayos, Cavia porcellus |
| Keyword: | Parasitology, Mammals, Viscera, Histopathology, Leishmania enriettii, Guinea pigs, Cavia porcellus |
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