Molecular characterization of Indian pathotypes of Puccinia striiformis f. sp. tritici and multigene phylogenetic analysis to establish inter- and intraspecific relationships
Título del documento: Molecular characterization of Indian pathotypes of Puccinia striiformis f. sp. tritici and multigene phylogenetic analysis to establish inter- and intraspecific relationships
Revue: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000424647
ISSN: 1415-4757
Autores: 1
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Instituciones: 1Indian Agricultural Research Institute, Fungal Molecular Biology Laboratory, Nueva Delhi, Delhi. India
2ICAR Indian Agricultural Research Institute, Regional Station Wellington, Tamil Nadu. India
3Indian Institute of Wheat and Barley Research, Regional Station, Shimla, Himachal Pradesh. India
Año:
Periodo: Dic
Volumen: 41
Número: 4
Paginación: 834-842
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés Abstract Stripe rust caused byPuccinia striiformisf. sp.tritici(Pst) is one of the most devastating diseases of wheat (Triticumspp.) worldwide. Indian isolates were characterised based on their phenotypic reaction on differential hosts carryingdifferentYrgenes. Based on virulence/avirulence structure, isolates were characterised into ten different pathotypesviz.70S0-2, 67S64, 70S4, 66S0, 70S64, 66S64-1, 38S102, 47S102, 46S119, and 78S84. These Indian pathotypesofP. striiformisf. sp.triticiand 38 pathotypes of other rust species (P. graminis triticiandP. triticina) were used in this study to analyze their molecular phylogenetic relationship. The nucleotides of rDNA-ITS, partialb-tubulinandketopantoate reductasegenes of all the pathotypes were sequenced directly after PCR. Based on sequence data ofrDNA-ITS andb-tubulin, three phylogenetic groups corresponding to three different species of Pucciniawere ob-tained. Asian isolates formed a distinct evolutionary lineage than from those derived from USA. The sequence similarity of Indian pathotypes with other Asian (China and Iran) isolates indicated the same origin of pathotypes. Theresults will allow rapid identification of Indian P.striiformisf. sp.triticipathotypes causing stripe rust in wheat, assist inmaking predictions regarding potential rust pathotypes, and identifying sources of resistance to the disease in advance
Disciplinas: Agrociencias,
Biología
Palabras clave: Fitopatología,
Hongos,
Evolución y filogenia,
Trigo,
Roya,
Virulencia,
Diversidad,
Filogenia
Keyword: Phytopathology,
Fungi,
Evolution and phylogeny,
Wheat,
Stripe rust,
Virulence,
Diversity,
Phylogeny
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