Molecular characterization of Indian pathotypes of Puccinia striiformis f. sp. tritici and multigene phylogenetic analysis to establish inter- and intraspecific relationships



Document title: Molecular characterization of Indian pathotypes of Puccinia striiformis f. sp. tritici and multigene phylogenetic analysis to establish inter- and intraspecific relationships
Journal: Genetics and molecular biology
Database: PERIÓDICA
System number: 000424647
ISSN: 1415-4757
Authors: 1
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Institutions: 1Indian Agricultural Research Institute, Fungal Molecular Biology Laboratory, Nueva Delhi, Delhi. India
2ICAR Indian Agricultural Research Institute, Regional Station Wellington, Tamil Nadu. India
3Indian Institute of Wheat and Barley Research, Regional Station, Shimla, Himachal Pradesh. India
Year:
Season: Dic
Volumen: 41
Number: 4
Pages: 834-842
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract Abstract Stripe rust caused byPuccinia striiformisf. sp.tritici(Pst) is one of the most devastating diseases of wheat (Triticumspp.) worldwide. Indian isolates were characterised based on their phenotypic reaction on differential hosts carryingdifferentYrgenes. Based on virulence/avirulence structure, isolates were characterised into ten different pathotypesviz.70S0-2, 67S64, 70S4, 66S0, 70S64, 66S64-1, 38S102, 47S102, 46S119, and 78S84. These Indian pathotypesofP. striiformisf. sp.triticiand 38 pathotypes of other rust species (P. graminis triticiandP. triticina) were used in this study to analyze their molecular phylogenetic relationship. The nucleotides of rDNA-ITS, partialb-tubulinandketopantoate reductasegenes of all the pathotypes were sequenced directly after PCR. Based on sequence data ofrDNA-ITS andb-tubulin, three phylogenetic groups corresponding to three different species of Pucciniawere ob-tained. Asian isolates formed a distinct evolutionary lineage than from those derived from USA. The sequence similarity of Indian pathotypes with other Asian (China and Iran) isolates indicated the same origin of pathotypes. Theresults will allow rapid identification of Indian P.striiformisf. sp.triticipathotypes causing stripe rust in wheat, assist inmaking predictions regarding potential rust pathotypes, and identifying sources of resistance to the disease in advance
Disciplines: Agrociencias,
Biología
Keyword: Fitopatología,
Hongos,
Evolución y filogenia,
Trigo,
Roya,
Virulencia,
Diversidad,
Filogenia
Keyword: Phytopathology,
Fungi,
Evolution and phylogeny,
Wheat,
Stripe rust,
Virulence,
Diversity,
Phylogeny
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