A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis
Título del documento: A cytosolic class II small heat shock protein, PfHSP17.2, confers resistance to heat, cold, and salt stresses in transgenic Arabidopsis
Revue: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000420038
ISSN: 1415-4757
Autores: 1
2
2
2
2
Instituciones: 1Zhejiang Sci-Tech University, School of Civil Engineering and Architecture, Zhenjiang. China
2Beijing Forestry University, College of Landscape Architecture, Beijing. China
Año:
Periodo: Sep
Volumen: 41
Número: 3
Paginación: 649-660
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés We cloned and characterized the full-length coding sequence of a small heat shock (sHSP) gene, PfHSP17.2 , from Primula forrestii leaves following heat stress treatment. Homology and phylogenetic analysis suggested that PfHSP17.2 is a cytosolic class II sHSP, which was further supported by the cytosolic localization of transient expres - sion of PfHSP17.2 fused with green fluorescent protein reporter. Expression analysis showed that PfHSP17.2 was highly inducible by heat stress in almost all the vegetative and generative tissues and was expressed under salt, cold, and oxidative stress conditions as well. Moreover, the expression of PfHSP17.2 in P. forrestii was detected in certain developmental growth stages. Transgenic Arabidopsis thaliana constitutively expressing PfHSP17.2 displayed increased thermotolerance and higher resistance to salt and cold compared with wild type plants. It is suggested that PfHSP17.2 plays a key role in heat and other abiotic stresses
Disciplinas: Biología,
Química
Palabras clave: Fisiología vegetal,
Genética,
Bioquímica,
Gen sHSPs,
Tolerancia al estrés,
Arabidopsis thaliana
Keyword: Plant physiology,
Genetics,
Biochemistry,
sHSPs gene,
Stress tolerance,
Arabidopsis thaliana
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