| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000420038 |
| ISSN: | 1415-4757 |
| Authors: | Lu Zhang1 Weijuan Hu2 Yike Gao2 Huitang Pan2 Qixiang Zhang2 |
| Institutions: | 1Zhejiang Sci-Tech University, School of Civil Engineering and Architecture, Zhenjiang. China 2Beijing Forestry University, College of Landscape Architecture, Beijing. China |
| Year: | 2018 |
| Season: | Sep |
| Volumen: | 41 |
| Number: | 3 |
| Pages: | 649-660 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | We cloned and characterized the full-length coding sequence of a small heat shock (sHSP) gene, PfHSP17.2 , from Primula forrestii leaves following heat stress treatment. Homology and phylogenetic analysis suggested that PfHSP17.2 is a cytosolic class II sHSP, which was further supported by the cytosolic localization of transient expres - sion of PfHSP17.2 fused with green fluorescent protein reporter. Expression analysis showed that PfHSP17.2 was highly inducible by heat stress in almost all the vegetative and generative tissues and was expressed under salt, cold, and oxidative stress conditions as well. Moreover, the expression of PfHSP17.2 in P. forrestii was detected in certain developmental growth stages. Transgenic Arabidopsis thaliana constitutively expressing PfHSP17.2 displayed increased thermotolerance and higher resistance to salt and cold compared with wild type plants. It is suggested that PfHSP17.2 plays a key role in heat and other abiotic stresses |
| Disciplines: | Biología, Química |
| Keyword: | Fisiología vegetal, Genética, Bioquímica, Gen sHSPs, Tolerancia al estrés, Arabidopsis thaliana |
| Keyword: | Plant physiology, Genetics, Biochemistry, sHSPs gene, Stress tolerance, Arabidopsis thaliana |
| Full text: | Texto completo (Ver PDF) |
