Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility
Título del documento: Xylanolytic enzymes production by Aspergillus niger GS1 from solid-state fermentation on corn stover and their effect on ruminal digestibility
Revue: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000336253
ISSN: 0717-3458
Autores: 1
1
1
2
3
1
Instituciones: 1Universidad Autónoma de Querétaro, Facultad de Química, Querétaro. México
2Centro de Investigación en Matemáticas, A.C., Guanajuato. México
3Universidad Autónoma de Querétaro, Facultad de Ciencias Naturales, Querétaro. México
Año:
Periodo: Mar
Volumen: 14
Número: 2
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Hemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzymes production, and their effect on in vitro ruminal and true digestibility of ACS. Enzyme production was empirically modelled using a fractional factorial design 29-5, and the resulting significant factors were glucose, yeast extract and two mineral salts, which were arranged in a Draper-Lin optimization design at two levels. Predicted optimum xylanolytic activity of 33.6 U (mg protein)-1 was achieved at 48 hrs of SSF, and was validated by confirmatory experiments. ACS was incubated with a semipurified enzymatic extract (EE) showing a xylanolytic activity of 1600 U kg-1 dry ACS for 12 hrs before exposure to cow’s ruminal liquid for 72 hrs, which led to 5% and 10% increase of in vitro ruminal and true digestibility, respectively. CS is a readily available by-product in different regions which after alkaline treatment and partial hydrolysis with the EE, may be advantageously used as supplement for ruminant feed
Disciplinas: Biología,
Medicina veterinaria y zootecnia
Palabras clave: Nutrición animal,
Biotecnología,
Rastrojo,
Maíz,
Fermentación en sustrato sólido,
Hidrólisis enzimática,
Xilanos,
Suplementos alimenticios,
Aspergillus niger
Keyword: Biology,
Veterinary medicine and animal husbandry,
Animal nutrition,
Biotechnology,
Stover,
Corn,
Solid state fermentation,
Enzymatic hydrolysis,
Xylans,
Food supplies,
Aspergillus niger
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