Revista: | Electronic journal of biotechnology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000336253 |
ISSN: | 0717-3458 |
Autores: | Regalado, Carlos1 Vázquez Obregón, Immer1 García Almendárez, Blanca Estela1 Domínguez Domínguez, Jorge2 Aguilera Barreyro, Araceli3 Amaro Reyes, Aldo1 |
Instituciones: | 1Universidad Autónoma de Querétaro, Facultad de Química, Querétaro. México 2Centro de Investigación en Matemáticas, A.C., Guanajuato. México 3Universidad Autónoma de Querétaro, Facultad de Ciencias Naturales, Querétaro. México |
Año: | 2011 |
Periodo: | Mar |
Volumen: | 14 |
Número: | 2 |
País: | Chile |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | Hemicellulosic agricultural by-products such as corn stover (CS) are highly available materials which represent an opportunity to develop value added products. Native Aspergillus niger GS1 was used for solid-state fermentation (SSF) on alkali pre-treated CS (ACS) aimed to optimize xylanolytic enzymes production, and their effect on in vitro ruminal and true digestibility of ACS. Enzyme production was empirically modelled using a fractional factorial design 29-5, and the resulting significant factors were glucose, yeast extract and two mineral salts, which were arranged in a Draper-Lin optimization design at two levels. Predicted optimum xylanolytic activity of 33.6 U (mg protein)-1 was achieved at 48 hrs of SSF, and was validated by confirmatory experiments. ACS was incubated with a semipurified enzymatic extract (EE) showing a xylanolytic activity of 1600 U kg-1 dry ACS for 12 hrs before exposure to cow’s ruminal liquid for 72 hrs, which led to 5% and 10% increase of in vitro ruminal and true digestibility, respectively. CS is a readily available by-product in different regions which after alkaline treatment and partial hydrolysis with the EE, may be advantageously used as supplement for ruminant feed |
Disciplinas: | Biología, Medicina veterinaria y zootecnia |
Palabras clave: | Nutrición animal, Biotecnología, Rastrojo, Maíz, Fermentación en sustrato sólido, Hidrólisis enzimática, Xilanos, Suplementos alimenticios, Aspergillus niger |
Keyword: | Biology, Veterinary medicine and animal husbandry, Animal nutrition, Biotechnology, Stover, Corn, Solid state fermentation, Enzymatic hydrolysis, Xylans, Food supplies, Aspergillus niger |
Texto completo: | Texto completo (Ver PDF) |