Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity
Título del documento: Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity
Revue: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451360
ISSN: 1984-8250
Autores: 1
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Instituciones: 1Universitas Indonesia, Faculty of Pharmacy, Depok, West Java. Indonesia
Año:
Volumen: 56
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés This study investigated the effect of ionic liquid (IL)-microwave assisted extraction (MAE) on γ-oryzanol extraction from rice bran (Oryza sativa L.). The conditions included the concentration of IL, liquid (IL)/solid (sample) ratio, extraction time, and microwave power. The use of 1-butyl-3-methylimidazolium ([Bmim]BF4) as a selected solvent in IL-MAE method was developed for extraction of γ-oryzanol from rice bran. The Box-Behnken design on four factors with response surface methodology (RSM) was used to optimize the experimental conditions. The optimum conditions for the MAE were a [Bmim]BF4 concentration of 0.7 M, liquid/solid ratio of 15 mL/g, extraction time of 10 minutes, and 30% microwave power with a γ-oryzanol value of 0.41 mg/g. The experimental values agreed with those predicted by the RSM models. A microplate reader method was used to determine the IC50 value in tyrosinase inhibitory activity of the extract. An IC50 value of the extract obtained from IL-MAE was 1240 µg/mL. This study suggests that IL-MAE is suitable for the extraction of γ-oryzanol from rice bran and that under the optimum conditions the extract has low activity as a tyrosinase inhibitor
Disciplinas: Química
Palabras clave: Fitoquímica,
Salvado de arroz,
Oryzanol,
Extracción química,
Extracción iónica líquida,
Inhibición enzimática,
Tirosinasa
Keyword: Phytochemistry,
Rice bran,
Oryzanol,
Chemical extraction,
Ionic liquid extraction,
Enzymatic inhibition,
Tyrosinase
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