Revista: | Brazilian Journal of Pharmaceutical Sciences |
Base de datos: | PERIÓDICA |
Número de sistema: | 000451360 |
ISSN: | 1984-8250 |
Autores: | Arrahman, Arif1 Sigalingging, Efendy1 Trinovita, Elsa1 Saputri, Fadlina Chany1 Mun'im, Abdul1 |
Instituciones: | 1Universitas Indonesia, Faculty of Pharmacy, Depok, West Java. Indonesia |
Año: | 2020 |
Volumen: | 56 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | This study investigated the effect of ionic liquid (IL)-microwave assisted extraction (MAE) on γ-oryzanol extraction from rice bran (Oryza sativa L.). The conditions included the concentration of IL, liquid (IL)/solid (sample) ratio, extraction time, and microwave power. The use of 1-butyl-3-methylimidazolium ([Bmim]BF4) as a selected solvent in IL-MAE method was developed for extraction of γ-oryzanol from rice bran. The Box-Behnken design on four factors with response surface methodology (RSM) was used to optimize the experimental conditions. The optimum conditions for the MAE were a [Bmim]BF4 concentration of 0.7 M, liquid/solid ratio of 15 mL/g, extraction time of 10 minutes, and 30% microwave power with a γ-oryzanol value of 0.41 mg/g. The experimental values agreed with those predicted by the RSM models. A microplate reader method was used to determine the IC50 value in tyrosinase inhibitory activity of the extract. An IC50 value of the extract obtained from IL-MAE was 1240 µg/mL. This study suggests that IL-MAE is suitable for the extraction of γ-oryzanol from rice bran and that under the optimum conditions the extract has low activity as a tyrosinase inhibitor |
Disciplinas: | Química |
Palabras clave: | Fitoquímica, Salvado de arroz, Oryzanol, Extracción química, Extracción iónica líquida, Inhibición enzimática, Tirosinasa |
Keyword: | Phytochemistry, Rice bran, Oryzanol, Chemical extraction, Ionic liquid extraction, Enzymatic inhibition, Tyrosinase |
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