Revue: | Brazilian archives of biology and technology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000346632 |
ISSN: | 1516-8913 |
Autores: | Tang, ZhenXing1 Qian, JunQing2 |
Instituciones: | 1Zhejiang University of Technology, College of Chemical Engineering and Materials Science, Zhejiang, Hangzhou. China 2Zhejiang University of Technology, College of Pharmaceutical Science, Zhejiang, Hangzhou. China |
Año: | 2007 |
Periodo: | Mar |
Volumen: | 50 |
Número: | 2 |
Paginación: | 299-309 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70% |
Disciplinas: | Química |
Palabras clave: | Bioquímica, Proteínas, Purificación química, Quitosano |
Keyword: | Chemistry, Biochemistry, Proteins, Chemical purification, Chitosan |
Texte intégral: | Texto completo (Ver HTML) |