| Revista: | Brazilian archives of biology and technology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000346632 |
| ISSN: | 1516-8913 |
| Autores: | Tang, ZhenXing1 Qian, JunQing2 |
| Instituciones: | 1Zhejiang University of Technology, College of Chemical Engineering and Materials Science, Zhejiang, Hangzhou. China 2Zhejiang University of Technology, College of Pharmaceutical Science, Zhejiang, Hangzhou. China |
| Año: | 2007 |
| Periodo: | Mar |
| Volumen: | 50 |
| Número: | 2 |
| Paginación: | 299-309 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. The optimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, low cross-linking rate, NaOH concentration 1.6%, amount of NaBH4 0.4 g. In order to use the chitosan gel, the elution conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of 2.03.0 mL/min. Particle size of chitosan gel was 120-140 µm. Neutral protease could be separated into four ingredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could be separated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70% |
| Disciplinas: | Química |
| Palabras clave: | Bioquímica, Proteínas, Purificación química, Quitosano |
| Keyword: | Chemistry, Biochemistry, Proteins, Chemical purification, Chitosan |
| Texto completo: | Texto completo (Ver HTML) |
