Embryonic stem-like cells derived from in vitro produced bovine blastocysts
Título del documento: Embryonic stem-like cells derived from in vitro produced bovine blastocysts
Revue: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000337634
ISSN: 1516-8913
Autores: 1
2
2
2
1
Instituciones: 1Universidade Federal de Goias, Instituto de Ciencias Biologicas, Goiania, Goias. Brasil
2Universidade Federal de Goias, Departamento de Producao Animal, Goiania, Goias. Brasil
Año:
Periodo: May-Jun
Volumen: 54
Número: 3
Paginación: 495-502
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico
Resumen en portugués The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1, respectively, were not expressed. Embryoid bodies were spontaneously formed at the seventh passage. Results showed that bovine ES-like cells could be obtained and passaged by mechanical procedures from the fresh in vitro produced blastocysts
Disciplinas: Medicina veterinaria y zootecnia
Palabras clave: Bovinos,
Genética,
Blastocisto,
Células madre,
Embriogénesis,
Marcadores genéticos
Keyword: Veterinary medicine and animal husbandry,
Bovines,
Genetics,
Blastocysts,
Embryogenesis,
Genetic markers,
Stem cells
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