Revista: | Brazilian archives of biology and technology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000337634 |
ISSN: | 1516-8913 |
Autores: | Freitas, Erika Regina Leal de1 Sanches, Bruno Valente2 Gambarini, Maria Lucia2 Oliveira-Filho, Benedito Dias2 Guillo, Lidia Andreu1 |
Instituciones: | 1Universidade Federal de Goias, Instituto de Ciencias Biologicas, Goiania, Goias. Brasil 2Universidade Federal de Goias, Departamento de Producao Animal, Goiania, Goias. Brasil |
Año: | 2011 |
Periodo: | May-Jun |
Volumen: | 54 |
Número: | 3 |
Paginación: | 495-502 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Analítico |
Resumen en portugués | The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1, respectively, were not expressed. Embryoid bodies were spontaneously formed at the seventh passage. Results showed that bovine ES-like cells could be obtained and passaged by mechanical procedures from the fresh in vitro produced blastocysts |
Disciplinas: | Medicina veterinaria y zootecnia |
Palabras clave: | Bovinos, Genética, Blastocisto, Células madre, Embriogénesis, Marcadores genéticos |
Keyword: | Veterinary medicine and animal husbandry, Bovines, Genetics, Blastocysts, Embryogenesis, Genetic markers, Stem cells |
Texto completo: | Texto completo (Ver HTML) |