Revue: | Anais da Academia Brasileira de Ciencias |
Base de datos: | PERIÓDICA |
Número de sistema: | 000400481 |
ISSN: | 0001-3765 |
Autores: | Cavalheiro, Mariana G1 Costa, Leandro Silva da1 Campos, Holmes S1 Alves, Leticia S2 Assuncao-Miranda, Iranaia2 Poian, Andrea T. da1 |
Instituciones: | 1Universidade Federal do Rio de Janeiro, Instituto de Bioquimica Medica Leopoldo de Meis, Rio de Janeiro. Brasil 2Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Goes, Rio de Janeiro. Brasil |
Año: | 2016 |
Periodo: | Sep |
Volumen: | 88 |
Número: | 3 |
Paginación: | 1485-1500 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | Alphaviruses among the viruses that cause arthritis, consisting in a public health problem worldwide by causing localized outbreaks, as well as large epidemics in humans. Interestingly, while the Old World alphaviruses are arthritogenic, the New World alphaviruses cause encephalitis. One exception is Mayaro virus (MAYV), which circulates exclusively in South America but causes arthralgia and is phylogenetically related to the Old World alphaviruses. Although MAYV-induced arthritis in humans is well documented, the molecular and cellular factors that contribute to its pathogenesis are completely unknown. In this study, we demonstrated for the first time that macrophages, key players in arthritis development, are target cells for MAYV infection, which leads to cell death through apoptosis. We showed that MAYV replication in macrophage induced the expression of TNF, a cytokine that would contribute to pathogenesis of MAYV fever, since TNF promotes an inflammatory profile characteristic of arthritis. We also found a significant increase in the production of reactive oxygen species (ROS) at early times of infection, which coincides with the peak of virus replication and precedes TNF secretion. Treatment of the cells with antioxidant agents just after infection completely abolished TNF secretion, indicating an involvement of ROS in inflammation induced during MAYV infection |
Disciplinas: | Medicina |
Palabras clave: | Virus, Bioquímica, Inmunología, Alfavirus, Virus Mayaro, Infecciones virales, Especies reactivas de oxígeno, Respuesta inflamatoria, Replicación viral |
Keyword: | Medicine, Virus, Biochemistry, Immunology, Alphavirus, Mayaro virus, Viral infections, Oxygen reactive species, Inflammatory response, Viral replication |
Texte intégral: | Texto completo (Ver HTML) |