Macrophages as target cells for Mayaro virus infection: involvement of reactive oxygen species in the inflammatory response during virus replication
Título del documento: Macrophages as target cells for Mayaro virus infection: involvement of reactive oxygen species in the inflammatory response during virus replication
Revista: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000400481
ISSN: 0001-3765
Autors: 1
1
1
2
2
1
Institucions: 1Universidade Federal do Rio de Janeiro, Instituto de Bioquimica Medica Leopoldo de Meis, Rio de Janeiro. Brasil
2Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Goes, Rio de Janeiro. Brasil
Any:
Període: Sep
Volum: 88
Número: 3
Paginació: 1485-1500
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Alphaviruses among the viruses that cause arthritis, consisting in a public health problem worldwide by causing localized outbreaks, as well as large epidemics in humans. Interestingly, while the Old World alphaviruses are arthritogenic, the New World alphaviruses cause encephalitis. One exception is Mayaro virus (MAYV), which circulates exclusively in South America but causes arthralgia and is phylogenetically related to the Old World alphaviruses. Although MAYV-induced arthritis in humans is well documented, the molecular and cellular factors that contribute to its pathogenesis are completely unknown. In this study, we demonstrated for the first time that macrophages, key players in arthritis development, are target cells for MAYV infection, which leads to cell death through apoptosis. We showed that MAYV replication in macrophage induced the expression of TNF, a cytokine that would contribute to pathogenesis of MAYV fever, since TNF promotes an inflammatory profile characteristic of arthritis. We also found a significant increase in the production of reactive oxygen species (ROS) at early times of infection, which coincides with the peak of virus replication and precedes TNF secretion. Treatment of the cells with antioxidant agents just after infection completely abolished TNF secretion, indicating an involvement of ROS in inflammation induced during MAYV infection
Disciplines Medicina
Paraules clau: Virus,
Bioquímica,
Inmunología,
Alfavirus,
Virus Mayaro,
Infecciones virales,
Especies reactivas de oxígeno,
Respuesta inflamatoria,
Replicación viral
Keyword: Medicine,
Virus,
Biochemistry,
Immunology,
Alphavirus,
Mayaro virus,
Viral infections,
Oxygen reactive species,
Inflammatory response,
Viral replication
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