| Journal: | Veterinaria México OA |
| Database: | PERIÓDICA |
| System number: | 000410536 |
| ISSN: | 2007-5472 |
| Authors: | Ibarra Zazueta, Cristina1 Arellano Reynoso, Beatriz1 Hernández Castro, Rigoberto2 Palomares Resendiz, Erika Gabriela3 Díaz Aparicio, Efrén3 |
| Institutions: | 1Universidad Nacional Autónoma de México, Facultad de Medicina Veterinaria y Zootecnia, Ciudad de México. México 2Hospital General Dr. Manuel Gea González, Departamento de Ecología y Agentes Patógenos, Ciudad de México. México 3Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Ciudad de México. México |
| Year: | 2016 |
| Season: | Oct-Dic |
| Volumen: | 3 |
| Number: | 4 |
| Pages: | 1-16 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Caseous lymphadenitis of small ruminants causes economic losses worldwide. To date, no effective vaccine has been developed against the causative agent of this disease, Corynebacterium pseudotuberculosis. The objective of the present work was to evaluate an aroA mutant gene strain of C. pseudotuberculosis in cellular and murine models, for attenuation and the ability to stimulate an immune response. The intracellular survival of the aroA mutant strain and the wild type strain (WT) of C. pseudotuberculosis was evaluated in J774A.1 murine macrophages using a multiplicity of infection (MOI) of 1:1 with the following infection times: 30 min, and 1, 2, 4, 8, 12, and 24 h. The largest difference in the intracellular survival of the mutant was observed 30 min post-infection. After subcutaneous skin vaccination, the subcutaneous lesion progression observed on the 14th day was more severe in those animals that were vaccinated with the WT strain. An analysis of the residual virulence in the murine model did not reveal any bacteria in mice vaccinated with the aroA strain on day 28 post-vaccination. Mice vaccinated with the mutant showed 50 % protection against the intraperitoneal challenge, exceeding that of the control group (41.67 %). We conclude that the virulence of the aroA mutant was significantly attenuated in both cellular and murine models according to the residual virulence detected in mice. However, vaccination with the mutant failed to confer at least 80 % protection, which is desirable for an immunogen. Hence, this study contributes to the knowledge of the immune response against Corynebacterium pseudotuberculosis |
| Disciplines: | Biología |
| Keyword: | Bacterias, Biología celular, Inmunología, Vacunas, Corynebacterium pseudotuberculosis |
| Keyword: | Biology, Bacteria, Cell biology, Immunology, Vaccines, Corynebacterium pseudotuberculosis |
| Full text: | Texto completo (Ver HTML) |
