| Journal: | Tropical and subtropical agroecosystems |
| Database: | PERIÓDICA |
| System number: | 000372734 |
| ISSN: | 1870-0462 |
| Authors: | Kawaka, J. Fanuel1 Kimenju, John W3 Ayodo, George1 Mwaniki, Shelmith W2 Muoma, John O1 Okoth, Sheila A3 Orinda, George O1 |
| Institutions: | 1Kenyatta University, Department of Biochemistry and Biotechnology, Nairobi. Kenia 2Kenya Agricultural Research Institute, National Agricultural Research Laboratories, Nairobi. Kenia 3University of Nairobi, Department of Plant Science and Crop Protection, Nairobi. Kenia |
| Year: | 2011 |
| Volumen: | 13 |
| Number: | 1 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Natural entomopathogenic nematodes (EPNs) are considered as potential biological control agents against soil-borne insect pests. This study was conducted to determine the impact of land use on the distribution, occurrence and diversity of entomopathogenic nematode community. Isolation of EPNs was done using the baiting technique and application of morphological identification methods revealed presence of the genus Steinernema. Land use intensification negatively affected the occurrence and recovery frequency in soils of Embu and Taita districts. The occurrence of EPNs was high in soils from coffee than maize and beans which had more nematodes than planted forest and napier grass followed by natural forest and tea respectively. PCR-RFLP of the internal transcribed spacer region on the ribosomal(r) DNA of the EPN isolates and digestion of the products by Alu I enzyme showed molecular variations among the isolates. The study has demonstrated that the frequency of occurrence and species variation of EPNs is different in various land uses |
| Disciplines: | Agrociencias, Biología |
| Keyword: | Suelos, Helmintos, Nemátodos entomopatógenos, Uso del suelo, Distribución espacial, Biodiversidad, Embu, Taita, Kenia |
| Keyword: | Agricultural sciences, Biology, Soils, Helminths, Land use, Spatial distribution, Biodiversity, Embu, Taita, Kenya |
| Full text: | Texto completo (Ver HTML) |
