| Journal: | The journal of venomous animals and toxins |
| Database: | PERIÓDICA |
| System number: | 000298688 |
| ISSN: | 0104-7930 |
| Authors: | Lima, P.R.M. de1 Brochetto-Braga, M.R Netto, J. Chaud |
| Institutions: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Instituto de Biociencias, Rio Claro, Sao Paulo. Brasil |
| Year: | 2000 |
| Volumen: | 6 |
| Number: | 1 |
| Pages: | 64-76 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Some properties of a Africanized honeybee venom proteases were determined by enzymatic assays in solution, electrophoresis in SDS-PAGE, and gel filtration. Bee venom extracts were obtained by reservoir disruption, selective dialysis (cut off 12 kDa) to eliminate small components, such as the protease inhibitor present in the venom, and then fractionation of the dialyzed extract by gel filtration on a Sephadex G-100 column. The optimal conditions for the caseinolytic assays were pH 9.5, 2-hour digestion at 37 °C, and 1% casein concentration. The proteolytic activity was also determined by electrophoresis in SDS-PAGE with co-polymerized gelatin with three major bands of 66.0, 41.6, and 25.1 kDa. A principal serine-protease-like mechanism was revealed in the enriched fraction of proteolytic activity |
| Disciplines: | Biología |
| Keyword: | Insectos, Bioquímica, Veneno de abeja, Abejas africanizadas, Actividad proteolítica, Cromatografía por filtración en gel, Apis mellifera, Hymenoptera |
| Keyword: | Biology, Insects, Biochemistry, Bee venom, Africanized bees, Proteolytic activity, Gel filtration chromatography, Apis mellifera, Hymenoptera |
| Full text: | Texto completo (Ver HTML) |
