| Journal: | The journal of venomous animals and toxins |
| Database: | PERIÓDICA |
| System number: | 000298618 |
| ISSN: | 0104-7930 |
| Authors: | Soares, A.M1 Anzaloni Pedrosa, L.H Fontes, M.R.M2 Silva, R.J. da3 Giglio, J.R |
| Institutions: | 1Universidade de Sao Paulo, Faculdade de Medicina, Ribeirao Preto, Sao Paulo. Brasil 2Universidade Estadual Paulista "Julio de Mesquita Filho", Departamento de Fisica e Biofisica, Botucatu, Sao Paulo. Brasil 3Universidade Estadual Paulista "Julio de Mesquita Filho", Centro de Estudos de Venenos e Animais Peconhentos, Botucatu, Sao Paulo. Brasil |
| Year: | 1998 |
| Volumen: | 4 |
| Number: | 2 |
| Pages: | 137-142 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Polyacrylamide gel electrophoresis (PAGE) for basic proteins may be a useful tool for the characterization of whole snake venoms and for the taxonomic classification of snakes of the Elapidae and Viperidae families. However, due to the close proximity of molecular weights among toxins bearing different net charges, sodium dodecyl sulfate (SDS) - PAGE was not able to provide an efficient differentiation. This article reports the electrophoretic analysis of several venoms from the genera Micrurus, Bothrops, Bothriopsis, Crotalus and Lachesis and shows a typical and distinctive electrophoretic profile for each species, with intraspecific and geographic variations. Even in cases in which extreme morphological similarities were present, such as between B. jararacussu and B. pirajai ("Bahia jararacuçu"), differentiation could be evidenced by PAGE. This simple and sensitive procedure may be applied to similar cases involving basic toxins |
| Disciplines: | Biología |
| Keyword: | Taxonomía y sistemática, Reptiles, Quimiotaxonomía, Veneno de víbora, Toxinas, Electroforesis en gel de poliacrilamida, Elapidae, Viperidae |
| Keyword: | Biology, Taxonomy and systematics, Reptiles, Chemotaxonomy, Snake venom, Toxins, Polyacrylamide gel electrophoresis, Elapidae, Viperidae |
| Full text: | Texto completo (Ver HTML) |
