| Journal: | The journal of venomous animals and toxins |
| Database: | PERIÓDICA |
| System number: | 000298767 |
| ISSN: | 0104-7930 |
| Authors: | Rahmy, T.R1 Ayoub, M.A2 |
| Institutions: | 1United Arab Emirates University, Faculty of Science, Al-Ain. Emiratos Arabes Unidos 2United Arab Emirates University, Faculty of Agricultural Sciences, Al-Ain. Emiratos Arabes Unidos |
| Year: | 2002 |
| Volumen: | 8 |
| Number: | 1 |
| Pages: | 127-159 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | Goat spermatozoa were incubated in vitro in Tris-citrate buffer, pH 7.2, containing 0, 40, 80, or 160 mug of Naja haje venom/mL buffer for 4 hours. During incubation, the percentages of sperm motility were decreased, while percentages of dead spermatozoa were increased in a time and concentration-dependent manner. The effect of venom concentrations on the ultrastructure of incubated spermatozoa was examined hourly by scanning (SEM) and transmission (TEM) electron microscopy. SEM results showed plasma membrane wrinkling at heads of some spermatozoa after 2 h incubation with 40mug venom. Most spermatozoa suffered membrane wrinkling after 4 h incubation. However, incubation with 80 mug venom caused membrane fractures in most sperm heads after 1 h incubation. The extent and depth of these fractures were increased after 2-3 h incubation. After 4 h incubation, plasma membrane focal erosion of many spermatozoa heads was common. Incubation with 160 mg venom induced sperm head swollen plasma membranes after 1 h incubation. Ruptured and disintegrated membranes were seen after 2 h; lysis and removal of external surface of spermatozoa head plasma membranes were recorded after 3-4 h incubation. TEM indicated slightly swollen areas on the sperm head plasma membrane, but showed normal nuclei, acrosomes, and tail regions after 2 h incubation in 40 mug cobra venom. The swollen areas were accompanied by sperm head membrane disintegration as well as membrane irregularities and distortion of tail mitochondrial cristae after 3-4 h incubation. However |
| Disciplines: | Medicina veterinaria y zootecnia |
| Keyword: | Medicina veterinaria, Ovinos y caprinos, Veneno de víbora, Cabras, Movilidad espermática, Ultraestructura celular, Naja haje, Elapidae |
| Keyword: | Veterinary medicine and animal husbandry, Sheep and goats, Veterinary medicine, Snake venom, Goats, Spermatozoa motility, Cell ultrastructure, Naja haje, Elapidae |
| Full text: | Texto completo (Ver HTML) |
