Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms



Document title: Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms
Journal: The journal of venomous animals and toxins including tropical diseases
Database: PERIÓDICA
System number: 000298950
ISSN: 1678-9199
Authors: 1







2

Institutions: 1Korea Food and Drug Administration, Department of Biologics Evaluation, Seúl. Corea del Sur
2Korea Food and Drug Administration, National Institute of Toxicological Research, Seúl. Corea del Sur
Year:
Volumen: 2006, vol.12, p.456-475
Number: 3
Pages: 456-475
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract To establish Korea National Standards for venoms and antivenoms, it is necessary to have standardized assay methods. In this study, we standardized a method to evaluate the antihemorrhagic potency of two horse-derived antivenoms using rabbit intracutaneous injection. We expressed the capability of these antivenoms to neutralize the hemorrhagic activities triggered by the venoms of Agkistrodon halys from Japan and Jiangzhe Agkistrodon halys from China as Minimum Hemorrhagic Dose (MHD). We also performed cross-neutralization tests employing the parallel line assay on different pairings of venoms and antivenoms to check the possibility of using Jiangzhe Agkistrodon halys venom as a substitute for the standard Agkistrodon halys venom in measurements of the antihemorrhagic activity, since A. halys venom is not easily available. Slope function ratio (S.R.) was 0.957 for Agkistrodon halys venom either with Agkistrodon halys antivenom or with Jiangzhe Agkistrodon halys antivenom (p>0.05). Similarly, S.R. was 0.348 for Jiangzhe Agkistrodon halys venom either with Agkistrodon halys antivenom or with Jiangzhe Agkistrodon halys antivenom (p>0.05). Thus, in this study we established antihemorrhagic potency test methods for both Agkistrodon halys and Jiangzhe Agkistrodon halys antivenoms and we could also show it is possible to use Jiangzhe Agkistrodon halys venom as a standard
Disciplines: Medicina
Keyword: Farmacología,
Toxicología,
Prueba de potencia,
Actividad antihemorrágica,
Antivenenos,
Agkistrodon halys,
Viperidae
Keyword: Medicine,
Pharmacology,
Toxicology,
Potency test,
Antihemorrhagic activity,
Antivenoms,
Agkistrodon halys,
Viperidae
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