| Journal: | The brazilian journal of infectious diseases |
| Database: | PERIÓDICA |
| System number: | 000290814 |
| ISSN: | 1413-8670 |
| Authors: | Melo, Sergio A.C1 Castro, Eduardo A.R2 Ponce de Leon, Antonio C3 Pereira, Jose A.A1 |
| Institutions: | 1Universidade do Estado do Rio de Janeiro, Faculdade de Ciencias Medicas, Rio de Janeiro. Brasil 2Universidade do Estado do Rio de Janeiro, Comissao de Controle de Infeccao Hospitalar, Rio de Janeiro. Brasil 3Universidade do Estado do Rio de Janeiro, Instituto de Medicina Social, Rio de Janeiro. Brasil |
| Year: | 2004 |
| Season: | Jun |
| Volumen: | 8 |
| Number: | 3 |
| Pages: | 190-196 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Caso clínico |
| English abstract | Bacterial colonization in hospitalized patients is an important step in nosocomial infections. Frequent employment of antimicrobials can modify patients' normal microbiota, favoring colonization and infection by antimicrobial-resistant microorganisms. First-generation cephalosporins are frequently used as prophylactic antibiotics in surgery. Intestinal, oropharyngeal and skin colonization by cephalothin-resistant microorganisms were studied in 60 pre-operative patients at the Hospital Universitário Pedro Ernesto. Feces were cultured in Eosin-methylene blue medium containing 32 µg/mL of cephalothin. Swabs obtained from the oropharynx and from skin were inoculated in cistein-lactose electrolytes-deficient medium containing 32 µg/mL of cephalothin. Isolated strains were identified and tested for susceptibility to antimicrobials by disk diffusion. Cephalothin-resistant strains were isolated from the feces of 59 patients (98%), from the oropharynx of 13 patients (22%) and from skin in 10 patients (17%). Enterobacter cloacae was predominant in feces (68% of the patients) and oropharynx (13%). Acinetobacter spp. was the most frequent microorganism isolated from the skin (10%). Antimicrobial multiresistant strains were isolated from at least one of the sites in 38 patients (63%). The employment of selective medium containing antimicrobials is a relatively simple and efficient method, being useful to evaluate microorganisms from hospitalized patients' microbiota that are relevant as potential pathogens in nosocomial infections |
| Disciplines: | Química, Medicina |
| Keyword: | Bioquímica, Diagnóstico, Microbiología, Medios selectivos, Cefalotina, Pacientes quirúrgicos |
| Keyword: | Chemistry, Medicine, Biochemistry, Diagnosis, Microbiology, Selective media, Cephalotine, Surgical patients |
| Full text: | Texto completo (Ver HTML) |
