| Revista: | The brazilian journal of infectious diseases |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000290824 |
| ISSN: | 1413-8670 |
| Autores: | Titze de Almeida, Ricardo1 Nogueira, Celeste A2 Rodrigues, Isabela P Ferreira, Renato F Eudes-Filho, Joao3 Moraes, Lidia M.P4 Soares Felipe, Maria Sueli Rollo-Filho, Mauricio5 Nascimento, Rejane S. do Boelens, Helene6 Van Belkum, Alex |
| Instituciones: | 1Universidade de Brasilia, Faculdade de Agronomia e Medicina Veterinaria, Brasilia, Distrito Federal. Brasil 2Hospital Universitario de Brasilia, Setor de Controle de Infeccao Microbiologia Molecular, Brasilia, Distrito Federal. Brasil 3Hospital Universitario de Brasilia, Laboratorio de Microbiologia, Brasilia, Distrito Federal. Brasil 4Instituto de Ciencias Biologicas, Laboratorio de Biologia Molecular, Brasilia, Distrito Federal. Brasil 5Universidade de Brasilia, Laboratorio de Bacteriologia da Saude Publica, Brasilia, Distrito Federal. Brasil 6Department of Medical Microbiology & Infectious Diseases, Rotterdam, Zuid Holland. Holanda |
| Año: | 2004 |
| Periodo: | Jun |
| Volumen: | 8 |
| Número: | 3 |
| Paginación: | 197-205 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | We studied the antimicrobial resistance and the molecular epidemiology of 99 enterococcal surveillance isolates from two hospitals of Brasília, Brazil. Conventional biochemical tests were used to identify the enterococcal species and the disk diffusion method was used to determine their resistance profiles. Enterococcus faecalis (76%) and E. faecium (9%) were the most prevalent species. No enterococci showed the vanA or vanB vancomycin resistance phenotypes or genotypes. Only the intrinsically resistant species E. gallinarum (n=2) and E. casseliflavus (n=3) harbored the vancomycin-resistance genes vanC1 and vanC2/3, respectively. We found E. faecalis isolates with high-level resistance to gentamicin (22%) and streptomycin (8%) and both E. faecalis and E. faecium isolates with resistance to more than two antimicrobials (84% and 67%, respectively). Nine E. faecalis isolates (12%) were resistant to ampicillin; the minimal inhibitory concentration (MIC) values were 16µg/mL (n=6) and 32µg/mL (n=3). Among these ampicillin-resistant E. faecalis, seven were also resistant to gentamicin, ciprofloxacin, rifampin, penicillin, chloramphenicol, tetracycline and erythromycin. Pulsed-field gel electrophoresis classified those isolates in three different genotypes, suggesting dissemination of genetically related ampicillin-resistant E. faecalis strains among different patients |
| Disciplinas: | Biología, Química |
| Palabras clave: | Bacterias, Genética, Bioquímica, Enterococos, Resistencia antimicrobiana, Genotipificación |
| Keyword: | Biology, Chemistry, Bacteria, Genetics, Biochemistry, Enterococci, Antimicrobial resistance, Genotyping |
| Texto completo: | Texto completo (Ver HTML) |
