| Journal: | The brazilian journal of infectious diseases |
| Database: | PERIÓDICA |
| System number: | 000291010 |
| ISSN: | 1413-8670 |
| Authors: | Macedo, Carla Isabel1 Carnieli-Junior, Pedro Brandao, Paulo Eduardo Rosa, Elizabeth S. Travassos da2 Oliveira, Rafael de Novaes Castilho, Juliana Galera Medeiros, Rita3 Machado, Rosangela Rocha4 Oliveira, Rosely Cerqueira de Carrieri, Maria Luiza Kotait, Ivanete |
| Institutions: | 1Instituto Pasteur, Sao Paulo. Brasil 2Instituto Evandro Chagas, Belem, Para. Brasil 3Hospital Universitario Joao de Barros Barreto, Belem, Para. Brasil 4Ministerio da Saude, Brasilia, Distrito Federal. Brasil |
| Year: | 2006 |
| Season: | Oct |
| Volumen: | 10 |
| Number: | 5 |
| Pages: | 341-345 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this same technique. Results were confirmed by DNA sequencing; this validates RT-PCR and neck-skin as a suitable technique and type of sample, respectively, for use in the diagnosis of human rabies. RT-PCR applied to neck-skin biopsies could allow early diagnosis and lead to more effective rabies treatment |
| Disciplines: | Biología, Química, Medicina |
| Keyword: | Virus, Bioquímica, Diagnóstico, Rabia humana, Piel del cuello, RT-PCR, Secuenciación del ADN |
| Keyword: | Biology, Chemistry, Medicine, Virus, Biochemistry, Diagnosis, Human rabies, Neck skin, RT-PCR, DNA sequencing |
| Full text: | Texto completo (Ver HTML) |
