Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay
Document title: Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay
Journal: The brazilian journal of infectious diseases
Database: PERIÓDICA
System number: 000290748
ISSN: 1413-8670
Authors: 1
2
Institutions: 1Universidade Federal de Sao Paulo, Divisao de Otorrinolaringologia Pediatrica, Sao Paulo. Brasil
2Universidade de Sao Paulo, Divisao de Doencas Infecciosas, Sao Paulo. Brasil
Year:
Season: Oct
Volumen: 7
Number: 5
Pages: 297-300
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental
English abstract In order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used simultaneously for culture, molecular assay and rapid GABHS antigen detection tests. All children were clinically examined at the Division of Pediatric Otorhinolaryngology of the Federal University of São Paulo. Diagnostic criteria were based on signs and symptoms, including sore throat, fever and oropharyngeal purulent secretion. Children that had been treated with antibiotics were excluded. Overall, combining the three methods, the prevalence of GABHS was 34%. GABHS was diagnosed in 30% of the bacterial cultures, in 25% of the samples tested with the molecular nucleic acid hybridization method and in 26% of the cases tested with the rapid antigen detection test. There was no significant difference between these three methods
Disciplines: Medicina,
Biología
Keyword: Diagnóstico,
Otorrinolaringología,
Bacterias,
Faringoamigdalitis,
Estreptococo beta hemolítico,
GABHS,
Detección de laboratorio
Keyword: Medicine,
Biology,
Diagnosis,
Otolaryngology,
Bacteria,
Acute pharyngotonsillitis,
Beta hemolytic streptococcus,
GABHS,
Laboratory detection
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