| Journal: | The brazilian journal of infectious diseases |
| Database: | PERIÓDICA |
| System number: | 000290870 |
| ISSN: | 1413-8670 |
| Authors: | De Paula, Sergio Oliveira1 Fonseca, Benedito Antonio Lopes da1 |
| Institutions: | 1Faculdade de Medicina de Ribeirao Preto, Departamento de Medicina Interna, Ribeirao Preto, Sao Paulo. Brasil |
| Year: | 2004 |
| Season: | Dic |
| Volumen: | 8 |
| Number: | 6 |
| Pages: | 390-398 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | Dengue is the most important disease caused by an arbovirus (1, 2, 3 and 4 serotypes) worldwide, especially in the tropical and sub-tropical regions. Its clinical manifestations range from asymptomatic infections to a severe disease characterized by hemorrhage and shock. The incidence of dengue virus activity in the Americas has substantially increased from 1980 to 1994. In Brazil, the increase in the incidence of dengue is especially linked to the dissemination of Aedes aegypti. Thus, a rapid and accurate dengue diagnosis is of paramount importance for effective control of dengue outbreaks [8]. Five serological tests have been used for the diagnosis of dengue infection: hemagglutination-inhibition (HI), complement fixation (CF), neutralization test (NT), immunoglobulin M (IgM) capture enzyme linked immunosorbent assay (MAC-ELISA) and indirect immunoglobulin G ELISA. The limitations of these techniques are the high cross-reactivity observed with these tests. Four methods of viral isolation have been routinely used for dengue viruses: intracerebral inoculation of newborn mice, inoculation on mammalian cell cultures, intrathoracic inoculation of adult mosquitoes, and inoculation on mosquito cell cultures. In recent years, several new diagnostic techniques have been developed and have proven very useful in dengue diagnosis, such as: nucleic and acid hybridization, RT-PCR. Currently, dengue diagnosis is based on serology, viral isolation and RNA detection. Enzyme-linked immunosorbent assays (ELISA) are still the most widely used techni |
| Disciplines: | Biología |
| Keyword: | Inmunología, Virus, Diagnóstico, Dengue, Serología, PCR |
| Keyword: | Biology, Immunology, Virus, Dengue, Serology, PCR, Diagnosis |
| Full text: | Texto completo (Ver HTML) |
