| Journal: | Summa phytopathologica |
| Database: | PERIÓDICA |
| System number: | 000285635 |
| ISSN: | 0100-5405 |
| Authors: | Santos, Elizama Roza1 Gouveia, Ester Ribeiro Souto-Maior, Ana Maria Mariano, Rosa Lima Ramos2 |
| Institutions: | 1Universidade Federal de Pernambuco, Departamento de Antibioticos, Recife, Pernambuco. Brasil 2Universidade Federal Rural de Pernambuco, Departamento de Agronomia, Recife, Pernambuco. Brasil |
| Year: | 2006 |
| Season: | Sep |
| Volumen: | 32 |
| Number: | 4 |
| Pages: | 376-378 |
| Country: | Brasil |
| Language: | Portugués |
| Document type: | Nota breve o noticia |
| Approach: | Experimental |
| English abstract | The bacterial fruit blotch, caused by the bacterium Acidovorax avenae subsp. citrulli (Aac), is responsible for great losses in melon production. The control of this disease was investigated in vivo by treating infected yellow melon seeds with fermented broths of B. subtilis R14, B. megaterium pv. cerealis RAB7, B. pumilus C116 and Bacillus sp. MEN2, with and without bacterial cells. The mechanism of action of the strains was studied in vitro by the agar diffusion technique. The bioactive compounds produced were partially characterized by hemolysis test and surfactant activity. Regarding the tests conducted in vivo there was no statistical difference between the treatments with and without bacterial cells, which indicated that the control was due to the presence of bioactive compounds produced during fermentations. All treatments were different from the control (P = 0.05%) but they did not differ among them. The B. megaterium pv. cerealis RAB7 reduced disease incidence (89.1%), disease index (92.7%), increased the incubation period from 9.8 to 11.9 days and reduced the area under disease progress curve from 3.36 to 0.17. In the in vitro tests, all strains showed antibiosis against Aac and the bioactive compounds were partially characterized as lipopeptides |
| Portuguese abstract | A mancha-aquosa, causada por Acidovorax avenae subsp. citrulli (Aac) causa grandes prejuízos à cultura do melão. O controle dessa doença foi estudado in vivo, com microbiolização de sementes de melão Amarelo infectadas, com líquidos fermentados de Bacillus subtilis R14, B. megaterium pv. cerealis RAB7, B. pumilus C116 e Bacillus sp. MEN2, com e sem células bacterianas. O mecanismo de ação dos isolados foi estudado in vitro pelo método de difusão em ágar e os compostos bioativos parcialmente caracterizados por testes de hemólise e atividade surfactante. Nos testes in vivo, não houve diferença significativa entre os tratamentos com e sem células, indicando que o controle ocorreu devido à presença de compostos bioativos produzidos durante as fermentações. Todos os tratamentos diferiram da testemunha sem diferir entre si (P=0,05%). B. megaterium pv. cerealis RAB7 proporcionou redução da incidência (89,1%) e do índice de doença (92,7%), elevou o período de incubação da mancha-aquosa de 9,8 para 11,9 dias e reduziu a AACPD de 3,36 para 0,17. In vitro, todos isolados apresentaram antibiose contra Aac e os compostos bioativos foram parcialmente caracterizados como lipopeptídeos |
| Disciplines: | Agrociencias |
| Keyword: | Fitopatología, Frutales, Acidovorax avenae, Control biológico, Bacillus subtilis, Melón, Cucumis melo |
| Keyword: | Agricultural sciences, Fruit trees, Phytopathology, Acidovorax avenae, Biological control, Bacillus subtilis, Melon, Cucumis melo |
| Full text: | Texto completo (Ver HTML) |
