Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022

Claro, Ingra Morales; Romano, Camila Malta; Candido, Darlan da Silva; Lima, Evelyn Lepka de; Lindoso, José Angelo Lauletta; Ramundo, Mariana Severo; Moreira, Filipe Romero Rebello; Barra, Luiz Alberto Costa; Borges, Luciana Marques Sansão; Medeiros, Lucas Alberto; Tomishige, Marcia Y. S.; Moutinho, Tomas; Silva, Anderson José Dias da; Rodrigues, Camila Cristina Martini; Azevedo, Luiz Cesar Fernandes de; Villas Boas, Lucy Santos; Silva, Camila Alves Maia da; Coletti, Thaís Moura; Manuli, Erika R.; O’Toole, Aine; Quick, Joshua; Loman, Nicholas; Rambaut, Andrew; Faria, Nuno R.; Figueiredo Mello, Claudia; Sabino, Ester Cerdeira


Document title:	Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022
Journal:	Revista do Instituto de Medicina Tropical de Sao Paulo
Database:
System number:	000547863
ISSN:	0036-4665
Authors:	Claro, Ingra Morales¹ Romano, Camila Malta¹ Candido, Darlan da Silva¹ Lima, Evelyn Lepka de⁴ Lindoso, José Angelo Lauletta¹ Ramundo, Mariana Severo¹ Moreira, Filipe Romero Rebello² Barra, Luiz Alberto Costa⁴ Borges, Luciana Marques Sansão⁴ Medeiros, Lucas Alberto⁴ Tomishige, Marcia Y. S.⁴ Moutinho, Tomas¹ Silva, Anderson José Dias da⁴ Rodrigues, Camila Cristina Martini⁴ Azevedo, Luiz Cesar Fernandes de⁴ Villas Boas, Lucy Santos¹ Silva, Camila Alves Maia da¹ Coletti, Thaís Moura¹ Manuli, Erika R.¹ O’Toole, Aine⁶ Quick, Joshua⁷ Loman, Nicholas⁷ Rambaut, Andrew⁶ Faria, Nuno R.¹ Figueiredo Mello, Claudia⁴ Sabino, Ester Cerdeira¹
Institutions:	¹Universidade de São Paulo, Faculdade de Medicina, São Paulo, São Paulo. Brasil ²Imperial College London, MRC Centre for Global Infectious Disease Analysis, London. Reino Unido ³University of Oxford, Department of Zoology, Oxford. Reino Unido ⁴Instituto de Infectologia Emílio Ribas, São Paulo, São Paulo. Brasil ⁵Universidade Federal do Rio de Janeiro, Instituto de Biologia, Rio de Janeiro, Rio de Janeiro. Brasil ⁶University of Edinburgh, Institute of Ecology and Evolution, Edinburgh. Reino Unido ⁷University of Birmingham, School of Biosciences, Birmingham. Reino Unido
Year:	2022
Volumen:	64
Country:	Brasil
Language:	Inglés
English abstract	Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.
Keyword:	Viral metagenomics, Monkeypox virus, West African clade, Genomic surveillance, Brazil
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Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022

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