| Journal: | Revista de investigación clínica |
| Database: | PERIÓDICA |
| System number: | 000454156 |
| ISSN: | 0034-8376 |
| Authors: | Adams Reyes, Nishi1 Coral Vázquez, Ramón M2 Méndez, Juan P1 Tenorio, Alberto3 Zenteno, Juan C1 Villegas Ruiz, Vanessa4 Canto, Patricia1 |
| Institutions: | 1Universidad Nacional Autónoma de México, Facultad de Medicina, Ciudad de México. México 2Instituto Politécnico Nacional, Escuela Superior de Medicina, Ciudad de México. México 3FUCAM, A.C., Instituto de Enfermedades de la Mama, Ciudad de México. México 4Instituto de Oftalmología "Fundación Conde de Valenciana", Departamento de Genética, Ciudad de México. México |
| Year: | 2019 |
| Season: | Jul-Ago |
| Volumen: | 71 |
| Number: | 4 |
| Pages: | 237-245 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Mitochondrial and oxidative stress has been related to obesity and breast cancer being this cancer more frequent and more aggressive in postmenopausal women with obesity. Objective The objective of this study was to investigate whether Mexican-Mestizo postmenopausal women with breast cancer and obesity present different somatic mutations in the mitochondrial DNA (mtDNA) when compared to women with normal body mass index (BMI). Subjects and Methods We included six Mexican-Mestizo postmenopausal women bearing breast cancer and who underwent mastectomy or breast-conserving surgery. BMI was determined in each case. Patients genomic DNA was isolated from blood leukocytes and tumor tissue samples. Whole mtDNA sequence was determined by MitoChip v2.0 mitochondrial resequencing array, and data were analyzed using the GeneChip Sequence Analysis Software. Tumor mtDNA sequence was compared with matched leukocyte mtDNA sequence. Results Three women had a normal BMI and three presented obesity. Overall, we found 64 genetic variants: 53.1% were somatic mutations and 46.9% were polymorphisms; 44.1% were in the non-coding region and 55.9% were in genes that encode for mitochondrial proteins. Among the somatic mutations, 67.7% were in patients with normal BMI and 32.3% in patients with obesity. Conclusions We did not find a higher frequency of mitochondrial somatic mutations in postmenopausal women with breast cancer and obesity compared to those with normal BMI. However, results could be due to the small number of women studied |
| Disciplines: | Medicina |
| Keyword: | Oncología, Genética, Ginecología y obstetricia, Genoma mitocondrial, Cáncer de mama, Postmenopausia, Indice de masa corporal, Mutación somática |
| Keyword: | Oncology, Genetics, Gynecology and obstetrics, Mitochondrial genome, Breast cancer, Postmenopause, Body mass index, Somatic mutation |
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