| Revista: | Revista de investigación clínica |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000455298 |
| ISSN: | 0034-8376 |
| Autores: | García Ruvalcaba, Azaria1 Vázquez Ibarra, Katia C1 Magaña Torres, María T1 Rizo de la Torre, Lourdes del C1 Meléndez Aranda, Lennon1 López Armas, Gabriela3 Cruz Ramos, José A4 Peregrina Sandoval, Jorge5 Espinoza Jiménez, Esther6 Rosales Gradilla, María E7 Sánchez López, Josefina Y1 |
| Instituciones: | 1Instituto Mexicano del Seguro Social, Centro de Investigación Biomédica de Occidente, Guadalajara, Jalisco. México 2Universidad de Guadalajara, Centro Universitario de Ciencias de la Salud, Guadalajara, Jalisco. México 3Centro de Educación Técnica Industrial, Guadalajara, Jalisco. México 4Instituto de Cancerología de Jalisco, Departamento de Biología Celular y Molecular, Guadalajara, Jalisco. México 5Universidad de Guadalajara, Centro Universitario de Ciencias Biológicas y Agropecuarias, Guadalajara, Jalisco. México 6Instituto Mexicano del Seguro Social, Hospital General Regional 110, Guadalajara, Jalisco. México 7Instituto Mexicano del Seguro Social, Centro Médico Nacional de Occidente, Guadalajara, Jalisco. México |
| Año: | 2023 |
| Periodo: | Ene-Feb |
| Volumen: | 75 |
| Número: | 1 |
| Paginación: | 37-44 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | Reduced or null expression of E-cadherin protein is a frequent cause of diffuse gastric cancer (DGC). More than 50% of patients with DGC present somatic variants in CDH1 gene. Objectives The objectives of this study were to study E-cadherin expression and identify variants in the CDH1 gene in gastric tumors of patients with DGC. Methods We studied 18 Mexican DGC patients who attended a hospital of the Mexican Social Security Institute; E-cadherin expression was determined by immunohistochemistry, and variants were identified by Sanger sequencing in promoter and coding regions. Predictive analysis was performed using PolyPhen-2 and HOPE software. Results We found that 56% of DGC patients showed reduced expression of E-cadherin. All patients carried CDH1 variants; overall, 12 different CDH1 variants were identified. Predictive analysis revealed that the rs114265540 variant was probably damaging, with a value of 0.985, indicating a functional impact on the E-cadherin protein. Variants rs34939176 and rs33964119 were identified as risk factors for DGC (odds' ratios [OR] = 31.3, 95% CI 6.3-154.0, p < 0.001; OR = 6.1, 95% CI 2.0-19.0, p < 0.001, respectively) given their elevated frequency and by comparing it with those reported for MXL population in the 1000 Genomes Project database. Conclusions In this Mexican population, the percentage of diffuse gastric tumors with reduced expression of E-cadherin was similar to that reported in other populations. All gastric tumors of DGC patients studied had somatic CDH1 gene variants; however, the rs114265540, rs34939176, and rs33964119 variants were importantly related to DGC |
| Disciplinas: | Medicina |
| Palabras clave: | Gastroenterología, Oncología, Cáncer gástrico, Tumores gástricos, Marcadores moleculares, Inmunohistoquímica, Cadherinas |
| Keyword: | Gastroenterology, Oncology, Gastric cancer, Gastric tumors, Molecular markers, Immunohistochemistry, Cadherins |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
