| Revista: | Revista Brasileira de Zootecnia |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000444036 |
| ISSN: | 1516-3598 |
| Autores: | Li, Sheng1 Chen, Chengzhen1 Jiang, Hao1 Zhang, Jiabao1 Zhou, Qian1 Gao, Yan1 Yuan, Bao1 Zhang, Mingjun1 |
| Instituciones: | 1Jilin University, College of Animal Sciences, Changchun, Jilin. China |
| Año: | 2019 |
| Volumen: | 48 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | In this study, our goal was to clarify the role of proteasomal subunit α-1 (PSMA1) in both the differentiation of preadipocytes and the accumulation of lipids in adipocytes. Preadipocytes from healthy one-day-old calves were collected, isolated, and cultured in vitro. The expression pattern of the PSMA1 gene was explored during the differentiation of bovine preadipocytes firstly. Then, the expression of the PSMA1 gene was inhibited by transfection of a chemically synthesized small interfering RNA (siRNA) before differentiation. After induction of differentiation, the mRNA levels of key regulating genes involved in preadipocyte differentiation and the lipid content of mature adipocytes with and without inhibition of PSMA1 were detected by qRT-PCR and oil red O staining, respectively. The data showed that PSMA1 mRNA was differentially expressed during the differentiation of bovine preadipocytes under normal culture conditions in vitro. The expression level of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT enhancer-binding protein alpha (C/EBPα), and lipoprotein lipase (LPL) were significantly decreased in the transfected PSMA1-siRNA group compared with those in the control group, and the mRNA levels of the preadipocyte factor-1 (Pref-1) were significantly upregulated in the transfected PSMA1-siRNA group compared with those in the control group. In addition, significantly fewer lipid droplets were formed by adipocytes transfected with PSMA1-siRNA than by the negative control group (adipocytes transfected with NC-siRNA). Therefore, PSMA1 plays an important role in differentiation and lipid deposition |
| Disciplinas: | Medicina veterinaria y zootecnia |
| Palabras clave: | Bovinos, Medicina veterinaria, Expresión génica, Depósito de grasa, Triglicéridos |
| Keyword: | Bovines, Veterinary medicine, Fat depot, Gene expression, Triglycerides |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
