Nucleopolyhedrovirus: scanning electron microscopy technique



Document title: Nucleopolyhedrovirus: scanning electron microscopy technique
Journal: Neotropical entomology
Database: PERIÓDICA
System number: 000299332
ISSN: 1519-566X
Authors: 1
2

3
Institutions: 1Universidade Estadual do Oeste do Parana, Laboratorio de Biologia Celular, Cascavel, Parana. Brasil
2Universidade Estadual de Maringa, Laboratorio de Anatomia Humana, Maringa, Parana. Brasil
3Universidade Estadual de Maringa, Departamento de Matematica, Maringa, Parana. Brasil
Year:
Season: Dic
Volumen: 35
Number: 6
Pages: 787-790
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the filtrate, inoculum and hemolymph of the silkworm experimentally infected with nuclear polyhedra. This material was placed on stubs, where a copper tape was previously adhered. After dry at room temperature the virus was covered with carbon and gold. Scanning electron microscopy analysis reveled a well defined morphology for the polyhedra of multiple Bombyx mori Nucleopolyhedrovirus, making possible the mathematical study that identified it as a truncated octahedron. The form of the polyhedron can present taxonomic value, once it is specific for each viral lineage
Portuguese abstract Um método simples foi desenvolvido para se estudar a forma geométrica do Bombyx mori Nucleopolyhedrovirus múltiplo, BmMNPV, para microscopia eletrônica de varredura. O vírus, pertencente à Família Baculoviridae, foi obtido do filtrado, do inóculo e da hemolinfa de lagartas de Bombyx mori (L.) (Lepidoptera: Bombycidae) infectadas. O material foi colocado diretamente sobre o suporte de varredura, onde previamente foi aderida uma fita de cobre. Após secagem e metalização com carbono e ouro, o vírus foi analisado ao microscópio de varredura. Os poliedros apresentaram-se com as faces lisas, perfeitamente definidas, possibilitando a análise matemática que o identificou como um octaedro truncado. A forma do poliedro pode apresentar valor taxonômico, uma vez que ela é específica para cada linhagem viral
Disciplines: Biología
Keyword: Insectos,
Taxonomía y sistemática,
Virus,
Octaedro truncado,
Baculovirus,
Bombyx mori,
Gusanos de seda,
BmMNPV,
Poliedro,
Microscopía electrónica de barrido
Keyword: Biology,
Insects,
Taxonomy and systematics,
Virus,
Truncated octahedron,
Baculovirus,
Bombyx mori,
Silkworms,
BmMNPV,
Polyhedra,
Scanning electron microscopy
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