| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000459440 |
| ISSN: | 0074-0276 |
| Authors: | Alves, Paula1 Emmel, Vanessa1 Stefanoff, Gustavo1 Krsticevic, Flavia1 Ezpeleta, Joaquín2 Murillo, Javier2 Tapia, Elizabeth2 Delatorre, Edson3 Abdelhay, Eliana1 Hassan, Rocio1 |
| Institutions: | 1Instituto Nacional do Cancer, Centro de Transplante de Medula Ossea, Rio de Janeiro. Brasil 2Universidad Nacional de Rosario, Centro Internacional Franco Argentino de Ciencias de la Información y de Sistemas, Rosario, Santa Fe. Argentina 3Universidade Federal do Espirito Santo, Centro de Ciencias da Saude, Vitoria, Espirito Santo. Brasil |
| Year: | 2023 |
| Volumen: | 118 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Epstein-Barr virus (EBV) is a human gammaherpesvirus etiologically linked to several benign and malignant diseases. EBV-associated malignancies exhibit an unusual global distribution that might be partly attributed to virus and host genetic backgrounds. OBJECTIVES To assemble a new genome of EBV (CEMO3) from a paediatric Burkitt’s lymphoma from Rio de Janeiro State (Southeast Brazil). In addition, to perform global phylogenetic analysis using complete EBV genomes, including CEMO3, and investigate the genetic relationship of some South American (SA) genomes through EBV subgenomic targets. METHODS CEMO3 was sequenced through next generation sequencing and its coverage and gaps were corrected through the Sanger method. CEMO3 and 67 EBV genomes representing diverse geographic regions were evaluated through maximum likelihood phylogenetic analysis. Further, the polymorphism of subgenomic regions of some SA EBV genomes were assessed. FINDINGS The whole bulk tumour sequencing yielded 23,217 reads related to EBV, which 172,713 base pairs of the newly EBV genome CEMO3 was assembled. The CEMO3 and most SA EBV genomes clustered within the SA subclade closely related to the African Raji strain, forming the South American/Raji clade. Notably, these Raji-related genomes exhibit significant genetic diversity, characterised by distinctive synapomorphies at some gene levels absent in the original Raji strain. CONCLUSION The CEMO3 represents a new South American EBV genome assembled. Albeit the majority of EBV genomes from SA are Raji-related, it harbours a high diversity different from the original Raji strain |
| Disciplines: | Medicina |
| Keyword: | Virus, Genética, Virus Epstein-Barr, Genoma viral, Diversidad genética, América del Sur |
| Keyword: | Virus, Genetics, Epstein-Barr virus, Viral genome, Genetic diversity, South America |
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