| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000401453 |
| ISSN: | 0074-0276 |
| Authors: | Pontoriero, Andrea1 Avaro, Martín1 Benedetti, Estefania1 Russo, Mara1 Czech, Andrea1 Periolo, Natalia1 Campos, Ana1 Zamora, Ana2 Baumeister, Elsa1 |
| Institutions: | 1Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán", Instituto Nacional de Enfermedades Infecciosas, Buenos Aires. Argentina 2Universidad Nacional de Tucumán, Facultad de Farmacia y Bioquímica, San Miguel de Tucumán, Tucumán. Argentina |
| Year: | 2016 |
| Season: | Dic |
| Volumen: | 111 |
| Number: | 12 |
| Pages: | 745-749 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Although vaccines are the best means of protection against influenza, neuraminidase inhibitors are currently the main antiviral treatment available to control severe influenza cases. One of the most frequent substitutions in the neuraminidase (NA) protein of influenza A(H3N2) viruses during or soon after oseltamivir administration is E119V mutation. We describe the emergence of a mixed viral population with the E119E/V mutation in the NA protein sequence in a post-treatment influenza sample collected from an immunocompromised patient in Argentina. This substitution was identified by a real-time reverse transcriptase polymerase chain reaction (RT-PCR) protocol and was confirmed by direct Sanger sequencing of the original sample. In 2014, out of 1140 influenza samples received at the National Influenza Centre, 888 samples (78%) were A(H3N2) strains, 244 (21.3%) were type B strains, and 8 (0.7%) were A(H1N1)pdm09 strains. Out of 888 A(H3N2) samples, 842 were tested for the E119V substitution by quantitative RT-PCR: 841 A(H3N2) samples had the wild-type E119 genotype and in one sample, a mixture of viral E119/ V119 subpopulations was detected. Influenza virus surveillance and antiviral resistance studies can lead to better decisions in health policies and help in medical treatment planning, especially for severe cases and immunocompromised patients |
| Disciplines: | Medicina |
| Keyword: | Microbiología, Neumología, Inmunología, Influenza, Virus de la influenza, Mutaciones, Resistencia a fármacos, Antivirales, Marcadores moleculares |
| Keyword: | Medicine, Microbiology, Pneumology, Immunology, Influenza, Influenza virus, Mutations, Drug resistance, Antiviral agents, Molecular markers |
| Full text: | Texto completo (Ver HTML) |
