Surveillance of antiviral resistance markers in Argentina: detection of E119V neuraminidase mutation in a post-treatment immunocompromised patient
Título del documento: Surveillance of antiviral resistance markers in Argentina: detection of E119V neuraminidase mutation in a post-treatment immunocompromised patient
Revista: Memorias do Instituto Oswaldo Cruz
Base de datos: PERIÓDICA
Número de sistema: 000401453
ISSN: 0074-0276
Autores: 1
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Instituciones: 1Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán", Instituto Nacional de Enfermedades Infecciosas, Buenos Aires. Argentina
2Universidad Nacional de Tucumán, Facultad de Farmacia y Bioquímica, San Miguel de Tucumán, Tucumán. Argentina
Año:
Periodo: Dic
Volumen: 111
Número: 12
Paginación: 745-749
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Although vaccines are the best means of protection against influenza, neuraminidase inhibitors are currently the main antiviral treatment available to control severe influenza cases. One of the most frequent substitutions in the neuraminidase (NA) protein of influenza A(H3N2) viruses during or soon after oseltamivir administration is E119V mutation. We describe the emergence of a mixed viral population with the E119E/V mutation in the NA protein sequence in a post-treatment influenza sample collected from an immunocompromised patient in Argentina. This substitution was identified by a real-time reverse transcriptase polymerase chain reaction (RT-PCR) protocol and was confirmed by direct Sanger sequencing of the original sample. In 2014, out of 1140 influenza samples received at the National Influenza Centre, 888 samples (78%) were A(H3N2) strains, 244 (21.3%) were type B strains, and 8 (0.7%) were A(H1N1)pdm09 strains. Out of 888 A(H3N2) samples, 842 were tested for the E119V substitution by quantitative RT-PCR: 841 A(H3N2) samples had the wild-type E119 genotype and in one sample, a mixture of viral E119/ V119 subpopulations was detected. Influenza virus surveillance and antiviral resistance studies can lead to better decisions in health policies and help in medical treatment planning, especially for severe cases and immunocompromised patients
Disciplinas: Medicina
Palabras clave: Microbiología,
Neumología,
Inmunología,
Influenza,
Virus de la influenza,
Mutaciones,
Resistencia a fármacos,
Antivirales,
Marcadores moleculares
Keyword: Medicine,
Microbiology,
Pneumology,
Immunology,
Influenza,
Influenza virus,
Mutations,
Drug resistance,
Antiviral agents,
Molecular markers
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