| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000459466 |
| ISSN: | 0074-0276 |
| Authors: | Rasinhas, Arthur da Costa1 Jácome, Fernanda Cunha1 Caldas, Gabriela Cardoso1 de Almeida, Ana Luisa Teixeira1 de Souza, Daniel Dias Coutinho1 dos Santos, João Paulo Rodrigues1 Dias, Helver Gonçalves1 Araujo, Eduarda Lima1 Mohana Borges, Ronaldo2 Barth, Ortrud Monika1 dos Santos, Flavia Barreto1 Barreto Vieira, Debora Ferreira1 |
| Institutions: | 1Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil 2Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Rio de Janeiro. Brasil |
| Year: | 2023 |
| Volumen: | 118 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | BACKGROUND Dengue is a disease caused by dengue virus (DENV-1 through -4). Among the four serotypes, DENV-4 remains the least studied. Acute kidney injury is a potential complication of dengue generally associated with severe dengue infection. OBJECTIVES The goal of this study was to investigate the alterations caused by experimental dengue infection in the kidney of adult BALB/c mice. METHODS In this study, BALB/c mice were infected through the intravenous route with a DENV-4 strain, isolated from a human patient. The kidneys of the mice were procured and subject to histopathological and ultrastructural analysis. FINDINGS The presence of the viral antigen was confirmed through immunohistochemistry. Analysis of tissue sections revealed the presence of inflammatory cell infiltrate throughout the parenchyma. Glomerular enlargement was a common find. Necrosis of tubular cells and haemorrhage were also observed. Analysis of the kidney on a transmission electron microscope allowed a closer look into the necrotic tubular cells, which presented nuclei with condensed chromatin, and loss of cytoplasm. MAIN CONCLUSIONS Even though the kidney is probably not a primary target of dengue infection in mice, the inoculation of the virus in the blood appears to damage the renal tissue through local inflammation |
| Disciplines: | Biología |
| Keyword: | Microbiología, Virus, Infección experimental, Virus del dengue, Ratones, Daño renal, Histopatología |
| Keyword: | Microbiology, Virus, Experimental infection, Dengue virus, Mice, Renal damage, Histopathology |
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