| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000459474 |
| ISSN: | 0074-0276 |
| Authors: | Andrade, Yasmin Monara Ferreira de Sousa1 Castro, Monara Viera de2 Tavares, Victor de Souza2 Souza, Rayane da Silva Oliveira2 Faccioli, Lúcia Helena3 Lima, Jonilson Berlink1 Sorgi, Carlos Arterio4 Borges, Valéria M1 Araújo Santos, Théo1 |
| Institutions: | 1Fundacao Oswaldo Cruz, Instituto Goncalo Moniz, Salvador, Bahia. Brasil 2Universidade Federal da Bahia, Centro das Ciencias Biologicas e da Saude, Barreiras, Bahia. Brasil 3Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil 4Universidade de Sao Paulo, Ribeirao Preto, Sao Paulo. Brasil |
| Year: | 2023 |
| Volumen: | 118 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | BACKGROUND The knowledge about eicosanoid metabolism and lipid droplet (LD) formation in the Leishmania is very limited and new approaches are needed to identify which bioactive molecules are produced of them. OBJECTIVES Herein, we compared LDs and eicosanoids biogenesis in distinct Leishmania species which are etiologic agents of different clinical forms of leishmaniasis. METHODS For this, promastigotes of Leishmania amazonensis, L. braziliensis and L. infantum were stimulated with polyunsaturated fatty acids (PUFA) and LD and eicosanoid production was evaluated. We also compared mutations in structural models of human-like cyclooxygenase-2 (GP63) and prostaglandin F synthase (PGFS) proteins, as well as the levels of these enzymes in parasite cell extracts. FINDINGS PUFAs modulate the LD formation in L. braziliensis and L. infantum. Leishmania spp with equivalent tissue tropism had same protein mutations in GP63 and PGFS. No differences in GP63 production were observed among Leishmania spp, however PGFS production increased during the parasite differentiation. Stimulation with arachidonic acid resulted in elevated production of hydroxyeicosatetraenoic acids compared to prostaglandins. MAIN CONCLUSIONS Our data suggest LD formation and eicosanoid production are distinctly modulated by PUFAS dependent of Leishmania species. In addition, eicosanoid-enzyme mutations are more similar between Leishmania species with same host tropism |
| Disciplines: | Medicina |
| Keyword: | Bioquímica, Protozoarios, Gotas lipídicas, Eicosanoides, Acidos grasos poliinsaturados, Biosíntesis, Leishmania |
| Keyword: | Biochemistry, Protozoa, Lipid droplet, Eicosanoids, Polyunsaturated fatty acids, Biosynthesis, Leishmania |
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