| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000459449 |
| ISSN: | 0074-0276 |
| Authors: | Miranda, Milene Dias1 Caldas, Gabriela Cardoso1 Ferreira, Vivian Neuza1 Barth, Ortrud Monika1 Silva, Aline de Paula Dias da1 Silva, Mayara Secco Torres3 Grinsztejn, Beatriz3 Veloso, Valdiléa Gonçalves3 Souza, Thiago Moreno2 Silva, Edson Elias da1 Barreto Vieira, Debora Ferreira1 |
| Institutions: | 1Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil 2Fundacao Oswaldo Cruz, Centro de Desenvolvimento Tecnologico em Saude, Rio de Janeiro. Brasil 3Fundacao Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Rio de Janeiro. Brasil |
| Year: | 2023 |
| Volumen: | 118 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Aplicado, descriptivo |
| English abstract | According to the last 2023 Monkeypox (Mpox) Outbreak Global Map from the Centres for Disease Control and Prevention (CDC), more than 100 countries with no Mpox infection report cases. Brazil stands out in this group and is the second country with the highest number of cases in the last outbreak. OBJECTIVE To contribute to knowledge of the virus infection effects in a cellular model, which is important for diagnosis infections not yet included in a provider´s differential diagnosis and for developing viral inhibition strategies. METHODS We describe a virus isolation protocol for a human clinical sample from a patient from Brazil, the viral growth in a cell model through plaque forming units (PFU) assay, reverse transcriptase polymerase chain reaction (RT-PCR) and transmission electron microscopy (TEM). FINDINGS We follow the viral isolation in Vero cell culture from a Mpox positive clinically diagnosed sample and show the infection effects on cellular structures using a TEM. MAIN CONCLUSIONS Understanding the impact of viral growth on cellular structures and its replication kinetics may offer better strategies for the development of new drugs with antiviral properties |
| Disciplines: | Biología |
| Keyword: | Virus, Microbiología, Viruela del mono, Aislamiento viral, Ultraestructura, Microscopía electrónica de transmisión |
| Keyword: | Virus, Microbiology, Monkey pox, Viral isolation, Ultrastructure, Transmission electron microscopy |
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