Revista: | Memorias do Instituto Oswaldo Cruz |
Base de datos: | PERIÓDICA |
Número de sistema: | 000400864 |
ISSN: | 0074-0276 |
Autores: | Villamizar, Luz Helena1 Cardoso, Maria das Gracas2 Andrade, Juliana de2 Teixeira, Maria Luisa2 Soares, Maurilio Jose1 |
Instituciones: | 1Fundacao Oswaldo Cruz, Instituto Carlos Chagas, Curitiba, Parana. Brasil 2Universidade Federal de Lavras, Departamento de Quimica, Lavras, Minas Gerais. Brasil |
Año: | 2017 |
Periodo: | Feb |
Volumen: | 112 |
Número: | 2 |
Paginación: | 131-139 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | Recent studies showed that essential oils from different pepper species (Piper spp.) have promising leishmanicidal and trypanocidal activities. OBJECTIVES In search for natural compounds against Trypanosoma cruzi, different forms of the parasite were incubated for 24 h at 28ºC or 4ºC with Piper aduncum essential oil (PaEO) or its main constituents linalool and nerolidol. METHODS PaEO chemical composition was obtained by GC-MS. Drug activity assays were based on cell counting, MTT data or infection index values. The effect of PaEO on the T. cruzi cell cycle and mitochondrial membrane potential was evaluated by flow cytometry. FINDINGS PaEO was effective against cell-derived (IC50/24 h: 2.8 μg/mL) and metacyclic (IC50/24 h: 12.1 μg/mL) trypomastigotes, as well as intracellular amastigotes (IC50/24 h: 9 μg/mL). At 4ºC - the temperature of red blood cells (RBCs) storage in blood banks - cell-derived trypomastigotes were more sensitive to PaEO (IC50/24 h = 3.8 μg/mL) than to gentian violet (IC50/24 h = 24.7 mg/mL). Cytotoxicity assays using Vero cells (37ºC) and RBCs (4ºC) showed that PaEO has increased selectivity for cell-derived trypomastigotes. Flow cytometry analysis showed that PaEO does not affect the cell cycle of T. cruzi epimastigotes, but decreases their mitochondrial membrane potential. GC-MS data identified nerolidol and linalool as major components of PaEO, and linalool had trypanocidal effect (IC50/24 h: 306 ng/mL) at 4ºC. MAIN CONCLUSION The trypanocidal effect of PaEO is likely due to the presence of linalool, which may represent an interesting candidate for use in the treatment of potentially contaminated RBCs bags at low temperature |
Disciplinas: | Química, Biología |
Palabras clave: | Fitoquímica, Protozoarios, Aceites esenciales, Actividad biológica, Linalool, Piper aduncum, Piperaceae, Trypanosoma cruzi |
Keyword: | Chemistry, Biology, Phytochemistry, Protozoa, Essential oils, Biological activity, Linalool, Piper aduncum, Piperaceae, Trypanosoma cruzi |
Texto completo: | Texto completo (Ver HTML) |