Heterogeneous persistence of Mycobacterium leprae in oral and nasal mucosa of multibacillary patients during multidrug therapy
Document title: Heterogeneous persistence of Mycobacterium leprae in oral and nasal mucosa of multibacillary patients during multidrug therapy
Journal: Memorias do Instituto Oswaldo Cruz
Database: PERIÓDICA
System number: 000452383
ISSN: 0074-0276
Authors: 1
2
3
3
1
1
Institutions: 1Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil
2Ministerio da Saude, Tecnologia e Insumos Estrategicos, Brasilia, Distrito Federal. Brasil
3Fundacao Oswaldo Cruz, Laboratorio de Biologia Molecular Aplicada a Micobacterias, Rio de Janeiro. Brasil
Year:
Volumen: 117
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract Leprosy is curable by multidrug therapy (MDT) treatment regimen ranging from six to 12 months. The variable levels of tolerance and adherence among patients can, however, result in treatment failure and the emergence of drug-resistant strains. OBJECTIVES Describe the impact of MDT over Mycobacterium leprae viability in patient’s oral and nasal mucosa along treatment. METHODS Mycobacterium leprae viability was monitored by quantitative polymerase chain reaction (qPCR) quantification of 16S rRNA in lateral and contralateral scrapings of oral and nasal mucosa of 10 multibacillary patients along the initial five months of treatment. FINDINGS The results demonstrated high heterogenicity of M. leprae viability among patients and between nasal and oral samples. Of six patients who presented good adherence and tolerance to the treatment, only four displayed absence of M. leprae viability in both samples three months after the first MDT dose, while for the other two, the absence of M. leprae viability in the oral and nasal cavities was only detected five months after the first dose. MAIN CONCLUSIONS We concluded that qPCR of 16S rRNA for the determination of M. leprae viability in nasal and oral scraping samples could represent an interesting approach to monitor treatment efficacy
Disciplines: Medicina
Keyword: Dermatología,
Farmacología,
Lepra,
Resistencia a fármacos,
Mycobacterium leprae
Keyword: Dermatology,
Pharmacology,
Leprosy,
Multidrug resistance,
Mycobacterium leprae
Full text: Texto completo (Ver HTML) Texto completo (Ver PDF)