| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000452383 |
| ISSN: | 0074-0276 |
| Autores: | Neumann, Arthur da Silva1 Fontes, Amanda Nogueira Brum2 Lopes, Márcia Quinhones Pires3 Suffys, Philip Noel3 Moraes, Milton Ozório1 Lara, Flávio Alves1 |
| Instituciones: | 1Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil 2Ministerio da Saude, Tecnologia e Insumos Estrategicos, Brasilia, Distrito Federal. Brasil 3Fundacao Oswaldo Cruz, Laboratorio de Biologia Molecular Aplicada a Micobacterias, Rio de Janeiro. Brasil |
| Año: | 2022 |
| Volumen: | 117 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Leprosy is curable by multidrug therapy (MDT) treatment regimen ranging from six to 12 months. The variable levels of tolerance and adherence among patients can, however, result in treatment failure and the emergence of drug-resistant strains. OBJECTIVES Describe the impact of MDT over Mycobacterium leprae viability in patient’s oral and nasal mucosa along treatment. METHODS Mycobacterium leprae viability was monitored by quantitative polymerase chain reaction (qPCR) quantification of 16S rRNA in lateral and contralateral scrapings of oral and nasal mucosa of 10 multibacillary patients along the initial five months of treatment. FINDINGS The results demonstrated high heterogenicity of M. leprae viability among patients and between nasal and oral samples. Of six patients who presented good adherence and tolerance to the treatment, only four displayed absence of M. leprae viability in both samples three months after the first MDT dose, while for the other two, the absence of M. leprae viability in the oral and nasal cavities was only detected five months after the first dose. MAIN CONCLUSIONS We concluded that qPCR of 16S rRNA for the determination of M. leprae viability in nasal and oral scraping samples could represent an interesting approach to monitor treatment efficacy |
| Disciplinas: | Medicina |
| Palabras clave: | Dermatología, Farmacología, Lepra, Resistencia a fármacos, Mycobacterium leprae |
| Keyword: | Dermatology, Pharmacology, Leprosy, Multidrug resistance, Mycobacterium leprae |
| Texto completo: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
