| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000459462 |
| ISSN: | 0074-0276 |
| Authors: | Vázquez, María Elisa1 Mesías, Andrea Cecilia1 Acuña, Leonardo1 Spangler, Joseph2 Zabala, Brenda1 Parodi, Cecilia1 Thakur, Meghna3 Oh, Eunkeu4 Walper, Scott Allan2 Brandán, Cecilia Pérez1 |
| Institutions: | 1Universidad Nacional de Salta, Instituto de Patología Experimental Dr. Miguel Angel Basombrío, Salta, Buenos Aires. Argentina 2United States Naval Academy, Center for Bio/Molecular Science & Engineering, Washington, Distrito de Columbia. Estados Unidos de América 3George Mason University, Fairfax, Virginia. Estados Unidos de América 4United States Naval Academy, Optical Science Division, Washington, Distrito de Columbia. Estados Unidos de América |
| Year: | 2023 |
| Volumen: | 118 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | BACKGROUND Vaccine development is a laborious craftwork in which at least two main components must be defined: a highly immunogenic antigen and a suitable delivery method. Hence, the interplay of these elements could elicit the required immune response to cope with the targeted pathogen with a long-lasting protective capacity. OBJECTIVES Here we evaluate the properties of Escherichia coli spherical proteoliposomes - known as outer membrane vesicles (OMVs) - as particles with natural adjuvant capacities and as antigen-carrier structures to assemble an innovative prophylactic vaccine for Chagas disease. METHODS To achieve this, genetic manipulation was carried out on E. coli using an engineered plasmid containing the Tc24 Trypanosoma cruzi antigen. The goal was to induce the release of OMVs displaying the parasite protein on their surface. FINDINGS As a proof of principle, we observed that native OMVs - as well as those carrying the T. cruzi antigen - were able to trigger a slight, but functional humoral response at low immunization doses. Of note, compared to the non-immunized group, native OMVs-vaccinated animals survived the lethal challenge and showed minor parasitemia values, suggesting a possible involvement of innate trained immunity mechanism. MAIN CONCLUSION These results open the range for further research on the design of new carrier strategies focused on innate immunity activation as an additional immunization target and venture to seek for alternative forms in which OMVs could be used for optimizing vaccine development |
| Disciplines: | Medicina |
| Keyword: | Farmacología, Inmunología, Vesículas de membrana externa, Escherichia coli, Vacunas, Trypanosoma cruzi |
| Keyword: | Pharmacology, Immunology, Outer membrane vesicles, Escherichia coli, Trypanosoma cruzi, Vaccines |
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