| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000401110 |
| ISSN: | 0074-0276 |
| Authors: | Coban, Ahmet Yilmaz1 Akbal, Ahmet Ugur1 Uzun, Meltem2 Cayci, Yeliz Tanriverdi1 Birinci, Asuman1 Durupinar, Belma1 |
| Institutions: | 1Ondokuz Mayis University Medical School, Department of Medical Microbiology, Samsun. Turquía 2Istanbul University, Istanbul Medical School, Estambul. Turquía |
| Year: | 2016 |
| Season: | Jul |
| Volumen: | 111 |
| Number: | 7 |
| Pages: | 454-459 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | In this study we evaluated the crystal violet decolorization assay (CVDA) for detection of minimum inhibitory concentration (MIC) of antituberculosis drugs. 53 isolates were tested in this study and 13 of them were multidrug resistant (MDR) isolates. The antibiotics concentrations were 2-0.06 mg/L for isoniazid (INH) and rifampicin (RIF) and were 16-0.25 mg/L for streptomycin (STM) and ethambutol (EMB). Crystal violet (CV-25 mg/L) was added into the microwells on the seventh day of incubation and incubation was continued until decolorization. Decolorization of CV was the predictor of bacterial growth. Overall agreements for four drugs were detected as 98.1%, and the average time was detected as 9.5 ± 0.89 day after inoculation. One isolate for INH and two isolates for STM were determined resistant in the reference method, but susceptible by the CVDA. One isolate was susceptible to EMB by the reference method, but resistant by the CVDA. All results were concordant for RIF. This study shows that CVDA is a rapid, reliable and suitable for determination of MIC values of Mycobacterium tuberculosis. And it can be used easily especially in countries with limited-sources |
| Disciplines: | Medicina |
| Keyword: | Farmacología, Microbiología, Fármacos antituberculosos, Pruebas de susceptibilidad, Resistencia a fármacos, Ensayo de decoloración de violeta cristal, Mycobacterium tuberculosis |
| Keyword: | Medicine, Microbiology, Pharmacology, Antituberculosis agents, Susceptibility tests, Drug resistance, Crystal violet decolorization assay, Mycobacterium tuberculosis |
| Full text: | Texto completo (Ver HTML) |
