Evaluation of crystal violet decolorization assay for minimal inhibitory concentration detection of primary antituberculosis drugs against Mycobacterium tuberculosis isolates
Título del documento: Evaluation of crystal violet decolorization assay for minimal inhibitory concentration detection of primary antituberculosis drugs against Mycobacterium tuberculosis isolates
Revista: Memorias do Instituto Oswaldo Cruz
Base de datos: PERIÓDICA
Número de sistema: 000401110
ISSN: 0074-0276
Autores: 1
1
2
1
1
1
Instituciones: 1Ondokuz Mayis University Medical School, Department of Medical Microbiology, Samsun. Turquía
2Istanbul University, Istanbul Medical School, Estambul. Turquía
Año:
Periodo: Jul
Volumen: 111
Número: 7
Paginación: 454-459
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés In this study we evaluated the crystal violet decolorization assay (CVDA) for detection of minimum inhibitory concentration (MIC) of antituberculosis drugs. 53 isolates were tested in this study and 13 of them were multidrug resistant (MDR) isolates. The antibiotics concentrations were 2-0.06 mg/L for isoniazid (INH) and rifampicin (RIF) and were 16-0.25 mg/L for streptomycin (STM) and ethambutol (EMB). Crystal violet (CV-25 mg/L) was added into the microwells on the seventh day of incubation and incubation was continued until decolorization. Decolorization of CV was the predictor of bacterial growth. Overall agreements for four drugs were detected as 98.1%, and the average time was detected as 9.5 ± 0.89 day after inoculation. One isolate for INH and two isolates for STM were determined resistant in the reference method, but susceptible by the CVDA. One isolate was susceptible to EMB by the reference method, but resistant by the CVDA. All results were concordant for RIF. This study shows that CVDA is a rapid, reliable and suitable for determination of MIC values of Mycobacterium tuberculosis. And it can be used easily especially in countries with limited-sources
Disciplinas: Medicina
Palabras clave: Farmacología,
Microbiología,
Fármacos antituberculosos,
Pruebas de susceptibilidad,
Resistencia a fármacos,
Ensayo de decoloración de violeta cristal,
Mycobacterium tuberculosis
Keyword: Medicine,
Microbiology,
Pharmacology,
Antituberculosis agents,
Susceptibility tests,
Drug resistance,
Crystal violet decolorization assay,
Mycobacterium tuberculosis
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