| Revista: | Memorias do Instituto Oswaldo Cruz |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000459469 |
| ISSN: | 0074-0276 |
| Autores: | Mendes Aguiar, Carolina de O1 Kitahara Oliveira, Milene Yoko1 Almeida, Ana Cristina Oliveira de2 Pereira Oliveira, Marcia1 Oliveira-Neto, Manoel Paes de3 Pirmez, Claude1 Sampaio, Elizabeth Pereira4 Gomes Silva, Adriano1 Da Cruz, Alda Maria1 |
| Instituciones: | 1Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil 2Universidade do Estado do Rio de Janeiro, Faculdade de Ciencias Medicas, Rio de Janeiro. Brasil 3Fundacao Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Rio de Janeiro. Brasil 4National Institute of Allergy and Infectious Diseases, Laboratory of Clinical Immunology and Microbiology, Bethesda, Maryland. Estados Unidos de América |
| Año: | 2023 |
| Volumen: | 118 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | BACKGROUND Dendritic cells (DCs) specific intercellular adhesion molecule (ICAM)-3-grabbing non integrin receptor (DC-SIGN) binds to subgenera Leishmania promastigotes mediating its interaction with DC and neutrophils, potentially influencing the infection outcome. OBJECTIVES In this work, we investigated whether DC-SIGN receptor is expressed in cells from cutaneous leishmaniasis (CL) lesions as well as the in vitro binding pattern of Leishmania (Viannia) braziliensis (Lb) and L. (L.) amazonensis (La) promastigotes. METHODS DC-SIGN receptor was labeled by immunohistochemistry in cryopreserved CL tissue fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mock-transfected (DC-SIGNNEG) were monitored by flow cytometry at 2 h, 24 h and 48 h in co-culture. RESULTS In CL lesion infiltrate, DC-SIGNPOS cells were present in the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while binding to DC-SIGNNEG was low. La showed precocious and higher affinity to DC-SIGNhi population than to DC-SIGNlow, while Lb binding was similar in these populations. CONCLUSION Our results demonstrate that DC-SIGN receptor is present in L. braziliensis CL lesions and interact with Lb promastigotes. Moreover, the differences in the binding pattern to Lb and La suggest DC-SIGN can influence in a difference way the intake of the parasites at the first hours after Leishmania infection. These results raise the hypothesis that DC-SIGN receptor could participate in the immunopathogenesis of American tegumentary leishmaniasis accounting for the differences in the outcome of the Leishmania spp. infection |
| Disciplinas: | Medicina |
| Palabras clave: | Diagnóstico, Inmunología, Leishmaniasis cutánea, Infiltrado inflamatorio, Receptores inmunológicos, Leishmania braziliensis, Leishmania amazonensis |
| Keyword: | Diagnosis, Immunology, Cutaneous leishmaniasis, Inflammatory infiltrate, Immunological receptors, Leishmania braziliensis, Leishmania amazonensis |
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